Effects of lactic acid bacteria fermentation on gel properties and flavor substances of lysozyme-free egg white

  • CHEN Jie ,
  • CHANG Cuihua ,
  • LI Junhua ,
  • SU Yujie ,
  • GU Luping ,
  • YANG Yanjun
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  • 1(State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(National Functional Food Engineering Technology Research Center, Jiangnan University, Wuxi 214122, China)
    3(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

Received date: 2022-01-29

  Revised date: 2022-03-03

  Online published: 2023-01-06

Abstract

This paper aims to promote the utilization of the lysozyme-free egg white (LFEW) solution after lysozyme extraction. The effects of different bacteria fermentation (Lactiplantibacillus plantarum LP90, Streptococcus thermophilus ST81, Lactobacillus bulgaricus LB42) on the desugarization, flavor and gel properties of LFEW were studied by determining the desugar rate, texture properties, water holding capacity (WHC), color difference, flavor substances. The experimental results showed that the removal of lysozyme could significantly improve the sugar removal rate of egg white in lactic acid bacteria fermentation. After fermentation treatment, the gel hardness of egg white reduced, the elasticity increased, the flavor was improved and a whiter color appeared. In comparison, the L. plantarum fermented LFEW gel showed the most appearing flavor. GC-MS identification showed that the contents of aromatic substances in the gel of LFEW fermented by L. plantarum decreased by 24.37%, while the total contents of alcohols and aldehyde increased by 10.29% and 10.89%, respectively. The main volatile substances were benzaldehyde, n-nonyl aldehyde and octanal. The study provides a new direction for the development and utilization of LFEW liquid.

Cite this article

CHEN Jie , CHANG Cuihua , LI Junhua , SU Yujie , GU Luping , YANG Yanjun . Effects of lactic acid bacteria fermentation on gel properties and flavor substances of lysozyme-free egg white[J]. Food and Fermentation Industries, 2022 , 48(24) : 30 -36 . DOI: 10.13995/j.cnki.11-1802/ts.031050

References

[1] GOSSETT P W, RIZVI S S H, BAKER R C.Quantitative analysis of gelation in egg protein systems[J].Food Technology, 1984, 38(5):67-96.
[2] NAU F, NYS Y, YAMAKAWA Y, et al.Nutritional value of the hen egg for humans[J].INRA Productions Animales, 2010, 23(2):225-236.
[3] WU T T, JIANG Q Q, WU D, et al.What is new in lysozyme research and its application in food industry? A review[J].Food Chemistry, 2019, 274:698-709.
[4] 侯宇. 蛋干生产工艺优化研究[D].长春:吉林大学, 2015.
HOU Y.Optimization of production process of egg dry[D].Changchun:Jilin University, 2015.
[5] 张强, 黄丽燕, 刘文营, 等.蛋白液鸡蛋干关键加工工艺研究[J].农产品加工, 2012(10):71-73;78.
ZHANG Q, HUANG L Y, LIU W Y, et al.Key process of egg curd using liquid egg white[J].Academic Periodical of Farm Products Processing, 2012(10):71-73;78.
[6] 李晶晶, 郑建仙.咸蛋蛋清液除腥脱盐的工艺[J].食品与发酵工业, 2009, 35(9):72-75.
LI J J, ZHENG J X.Study on deodorization and desalination for saline egg white[J].Food and Fermentation Industries, 2009, 35(9):72-75.
[7] 卢嘉, 林菲, 卢晓明, 等.全蛋液脱腥工艺及其效果评价[J].中国食品学报, 2017, 17(3):171-176.
LU J, LIN F, LU X M, et al.Deodorization process of whole liquid egg and effectiveness evaluation[J].Journal of Chinese Institute of Food Science and Technology, 2017, 17(3):171-176.
[8] 魏明英, 胡太健.HACCP在鸡蛋干生产中的应用[J].食品研究与开发, 2016, 37(16):198-201.
WEI M Y, HU T J.Application of HACCP system in egg curd rocessing[J].Food Research and Development, 2016, 37(16):198-201.
[9] LI J H, WANG J, ZHAI J L, et al.Improving gelling properties of diluted whole hen eggs with sodium chloride and sodium tripolyphosphate:Study on intermolecular forces, water state and microstructure[J].Food Chemistry, 2021, 358:129823.
[10] LI J H, LI X, WANG C Y, et al.Characteristics of gelling and water holding properties of hen egg white/yolk gel with NaCl addition[J].Food Hydrocolloids, 2018, 77:887-893.
[11] DAI Y, ZHAO J, GAO J, et al.Heat-and cold-induced gels of desalted duck egg white/gelatin mixed system:Study on rheological and gel properties[J].Food Hydrocolloids, 2021, 121:107003.
[12] 陈正. 微波法快速制备蛋黄风味基料及其风味特性的研究[D].无锡:江南大学, 2020.
CHEN Z.Characterization of egg yolk flavoring fast prepared by microwave irradiation[D].Wuxi:Jiangnan University, 2020.
[13] 董园园, 李睿佳, 张明.基于气相色谱-嗅闻-质谱联用技术和香气活度值解析及调控鸡蛋干关键风味物质[J].食品与发酵工业, 2020, 46(16):220-225.
DONG Y Y, LI R J, ZHANG M.GC-O-MS and OAV based analysis of key flavor substances and their regulation in egg curd[J].Food and Fermentation Industries, 2020, 46(16):220-225.
[14] LESNIEROWSKI G, KIJOWSKI J.Lysozyme[M].UBerlin, Heidelberg:Springer Berlin Heidelberg, 2007.
[15] SHIMAZAKI Y, TAKAHASHI A.Antibacterial activity of lysozyme-binding proteins from chicken egg white[J].Journal of Microbiological Methods, 2018, 154:19-24.
[16] JOHNSON T M, ZABIK M E.Gelation properties of albumen proteins, singly and in combination[J].Poultry Science, 1981, 60(9):2 071-2 083.
[17] IWASHITA K, HANDA A, SHIRAKI K.Co-aggregation of ovotransferrin and lysozyme[J].Food Hydrocolloids, 2019, 89:416-424.
[18] JIANG Y W, JIA J, XIONG D D, et al.Effects of short-term fermentation with lactic acid bacteria on egg white:Characterization, rheological and foaming activities[J].Food Hydrocolloids, 2020, 101:105507.
[19] TIAN L J, HU S T, JIA J, et al.Effects of short-term fermentation with lactic acid bacteria on the characterization, rheological and emulsifying properties of egg yolk[J].Food Chemistry, 2021, 341:128163.
[20] LEE W C, CHEN T C.Effects of drying temperature and desugarization on gelation characteristics of egg white solids[J].Journal of Food Processing and Preservation, 1999, 23(4):349-353.
[21] LI J H, YANG Y J, SU Y J, et al.Phase separation in ternary composite cold-set gel of egg yolk/κ-carrageenan/xanthan:Study on rheological and proton mobility properties[J].LWT, 2019, 116:108497.
[22] 陈静慧, 石浩, 张强, 等.基于电子鼻和顶空固相微萃取-气相质谱联用技术分析柠檬草中的挥发性成分[J].食品与发酵工业, 2019, 45(3):231-236.
CHEN J H, SHI H, ZHANG Q, et al.Analysis and discrimination of volatile compounds of Cymbopogon citratus by electronic nose combined with HS-SPME-GC-MS[J].Food and Fermentation Industries, 2019, 45(3):231-236.
[23] 张礼欣, 于小聪, 王泽琨, 等.基于气味信息的荆芥穗产地鉴别[J].中国药房, 2021, 32(18):2 203-2 209.
ZHANG L X, YU X C, WANG Z K, et al.Identification of the origin of schizonepeta tenusfolia based on"Odor"information[J].China Pharmacy, 2021, 32(18):2 203-2 209.
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