Food and Fermentation Industries >

2022 , Vol. 48 >Issue 24: 220 - 225

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030165

Repair of UVB light damage in HaCaT cells by Ganoderma lucidum bidirectional fermentation broth

  • ZHANG Ying ,
  • MENG Xianyao ,
  • YANG Yunli ,
  • GUO Miaomiao ,
  • ZHOU Weiqiang ,
  • LI Li
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  • 1(College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing 100048, China)
    2(Beijing Key Lab of Plant Resource Research and Development, Beijing 100048, China)
    3(Nutrition & Health Research Institute, COFCO Corporation, Beijing 102209, China)

Received date: 2021-11-29

  Revised date: 2022-03-26

  Online published: 2023-01-06

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Abstract

The aim of this study is to explore the repair effects of Ganoderma lucidum, G. lucidum-Polygonatum odoratum, G. lucidum-Inonotus obliquus, G. lucidum-Panax ginseng and G. lucidum-Panax notoginseng fermentation broth freeze-dried powder on human keratinocytes (HaCaT) photodamage induced by UVB, and to screen out the optimal fermentation combination of G. lucidum. Lyophilized powder of G. lucidum, G. lucidum-P. odoratum was prepared by liquid deep fermentation. MTT cytotoxicity assay and cell morphology were used to screen out the concentration of the sample. The damage model of HaCaT cells under 300 mJ/cm2 UVB radiation was established. The levels of interleukin-1α (IL-1α) and prostaglandinE2 (PGE2) secreted by HaCaT cells were determined by enzyme linked immunosorbent assay (ELISA). The results showed that compared with the lyophilized powder of G. lucidum fermentation broth, the lyophilized powder of G. lucidum-I. obliquus fermentation had significant inhibitory effect on the secretion of IL-1α and PGE2 (P<0.05). Therefore, this paper concluded that compared with the lyophilized powder of G. lucidum fermentation liquid, the lyophilized powder of G. lucidum-I. obliquus fermentation liquid has the strongest ability to repair UVB light damage of HaCaT cells. Therefore, the optimal fermentation combination method of G. lucidum-I. obliquus fermentation is the best, which provides the premise and basis for the industrial production of G. lucidum fermentation combination.

Key words： Ganoderma lucidum; fermentation; HaCaT cells; UVB rays; photodamage

Cite this article

ZHANG Ying , MENG Xianyao , YANG Yunli , GUO Miaomiao , ZHOU Weiqiang , LI Li . Repair of UVB light damage in HaCaT cells by Ganoderma lucidum bidirectional fermentation broth[J]. Food and Fermentation Industries, 2022 , 48(24) : 220 -225 . DOI: 10.13995/j.cnki.11-1802/ts.030165

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