An ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-MS/MS) method was developed and optimized for the simultaneous determination of 36 preservatives in protein drinks and liquid condiments by the pretreatment method of combining extraction and purification. The samples were purified with saturated sodium chloride solution (adjusting pH to 3 with phosphoric acid) and extracted with acetonitrile-methanol (9∶1, volume ratio) (containing 0.2% formic acid). The analytes were separated on a C18 chromatographic column using gradient elution with methanol-5 mmol/L ammonium acetate as the mobile phase, detected by MS/MS in both positive ion and negative ion mode using dynamic multiple reaction monitoring, and finally quantified by the matrix-matched external standard method. Results showed that good linearity was observed for the 36 compounds with correlation coefficients≥0.999 in the concentration range of 1-250 ng/mL, and the limits of quantitation were between 0.04 mg/kg and 0.2 mg/kg. The average recoveries of blank samples were in the range of 75%-119%, with relative standard deviations of 0.90%-9.8%. The established high-throughput detection method is sensitive, rapid, accurate, and simple, can effectively reduce matrix interference, reduce detection cost, shorten the detection cycle, and greatly improve the qualitative and quantitative detection efficiency of various preservatives.
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