Food and Fermentation Industries >

2024 , Vol. 50 >Issue 2: 23 - 33

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034346

Extraction of polysaccharides from Urtica fissa and the hypoglycemic activity on HepG2 cells

  • YANG Wenjuan ,
  • SHUAI Zhaorui ,
  • GONG Pin ,
  • YAO Wenbo ,
  • LI Nan ,
  • ZHANG Jiayuan ,
  • CHENG Shirui
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  • (School of Food Science and Engineering, Shaanxi University of Science & Technology, Xi′an 710021, China)

Received date: 2022-11-18

  Revised date: 2023-01-17

  Online published: 2024-02-27

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Abstract

To study the hypoglycemic effect of polysaccharides from Urtica fissa (PUF) on HepG2 cells, the semi-biomimetic extraction process of PUF was optimized by single-factor experiments and response surfaces using Urtica fissa as raw materials.The hypoglycemic effect of PUF was determined by α-glucosidase and α-amylase inhibition experiments.The hypoglycemic effect of PUF was explored by constructing an insulin-resistant HepG2 cell (IR-HepG2) model and measuring the contents of triglyceride (TG), hexokinase (HK), pyruvate kinase (PK), and glycogen.Results showed that the yield of PUF was 7.04% when the pH1 was 3, the pH2 was 8, the solid-liquid ratio was 1∶16 (g∶mL), the extraction temperature was 65 ℃, and the extraction time was 75 min.α-Glucosidase and α-amylase inhibition experiments showed that PUF could exhibit hypoglycemic effects by inhibiting α-glucosidase and α-amylase.Cell experiments showed that PUF could reduce insulin resistance by affecting glucose consumption in IR-HepG2 cells, promote glycogen synthesis, and reduce TG content to regulate glycolipid metabolism by increasing the content of HK and PK, thereby playing a hypoglycemic effect.In summary, PUF has strong hypoglycemic activity, which provides a theoretical basis for its application to auxiliary functional foods for diabetic patients.

Key words： polysaccharides from Urtica fissa; process optimization; HepG2 cells; hypoglycemic effect

Cite this article

YANG Wenjuan , SHUAI Zhaorui , GONG Pin , YAO Wenbo , LI Nan , ZHANG Jiayuan , CHENG Shirui . Extraction of polysaccharides from Urtica fissa and the hypoglycemic activity on HepG2 cells[J]. Food and Fermentation Industries, 2024 , 50(2) : 23 -33 . DOI: 10.13995/j.cnki.11-1802/ts.034346

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