Food and Fermentation Industries >

2024 , Vol. 50 >Issue 4: 102 - 109

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035340

Enzyme activity in pit mud with different position and its correlation with bacterial community structure

  • ZENG Bo ,
  • PU Jizhou ,
  • CHEN Qiuxu ,
  • RAO Jiaquan ,
  • ZOU Yongfang ,
  • WEN Jing ,
  • HUANG Zhiguo ,
  • WEI Chunhui
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  • 1(Liquor Brewing Biotechnology and Application Key Laboratory of Sichuan Province, Sichuan University of Science & Engineering, Yibin 644000, China)
    2(Shede Spirits Co.Ltd., Shehong 629000, China)
    3(Liquor Brewing Biotechnology and Intelligent Manufacturing Key Laboratory of China Light Industry, Yibin 644000, China)

Received date: 2023-03-02

  Revised date: 2023-03-22

  Online published: 2024-03-15

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Abstract

The Baijiu quality was affected by the enzymatic reaction of pit mud microorganisms. At present, there was little research on pit mud enzyme activity and spatial distribution characteristics of nitrogen metabolism enzymes in pit mud. The study analyzed physicochemical indicators and enzyme activities of pit mud at different locations. High-throughput sequencing was used to elaborate the bacterial communities. Cluster analysis was used to explore the distribution characteristics of the bacterial community from the view of space-time. Gene sequence of pit mud microorganism was used to predict the relative abundance of enzymes which involved in nitrogen metabolism. The results showed that ammonium nitrogen content (1 478.37 mg/kg), catalase activity [1.731 mg/(g·d)] and protease activity [1.107 mg/(g·d)] increased from top to bottom in the pit, while dehydrogenase activity decreased. Firmicutes was the absolute dominant bacteria of pit mud. Lactobacillus, Caproiciproduces, and Fastidiosipila were the first dominant bacteria in bottom pit mud, wall mezzanine pit mud, and wall top pit mud, respectively. Ammonium nitrogen, protease, and Aminobacterium showed significant positive correlation (P<0.05). Acid phosphatase was significantly negatively correlated with Lactobacillus (P<0.05), urease was significantly positively correlated with Synthephaceticus (P<0.05). In the function prediction of PICRUSt2, the relative abundance of functional enzymes that involves in nitrogen metabolism in pit mud is significantly different at different locations (P＜0.05). Enzyme activity affected pit mud material circulation. Material exchange was an important driving force for microbial interaction. Functional enzyme activity could measure the intensity of nitrogen metabolism. It can be seen that pit mud location had an important impact on the distribution of nitrogen metabolizing enzymes and nitrogen metabolism. The study provides a theoretical basis for analyzing the participation of microorganisms in the material circulation process in pit mud.

Key words： different locations; pit mud; enzyme activity; bacterial community; correlation

Cite this article

ZENG Bo , PU Jizhou , CHEN Qiuxu , RAO Jiaquan , ZOU Yongfang , WEN Jing , HUANG Zhiguo , WEI Chunhui . Enzyme activity in pit mud with different position and its correlation with bacterial community structure[J]. Food and Fermentation Industries, 2024 , 50(4) : 102 -109 . DOI: 10.13995/j.cnki.11-1802/ts.035340

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