For large-scale production of papain using aqueous two-phase extraction (ATPE), high extractant concentrations and volume ratio are usually required to obtain high recovery, but this results in a decrease in the purity and concentration of the target. In this study, the aqueous two-phase system composed of polyethylene glycol (PEG) and sodium citrate (Na3C6H5O7) was transferred between multiple centrifuge tubes to simulate the multi-stage crosscurrent extraction and multi-stage countercurrent extraction processes commonly used in industry, and the theoretical number of stages of multi-stage countercurrent ATPE was obtained by McCabe Thiele diagram method with the indexes of partition coefficient and recovery of enzyme activity. The results showed that the recovery of papain activity using the three-stage countercurrent ATPE achieved 96.75% when the volume radio was 0.5 and the initial concentration of papain in the bottom phase of the feedstock was 0.20 mg/mL, while an optimized scheme for multi-stage countercurrent ATPE of papain was designed.
ZHU Hengwei
,
BAI Xinpeng
,
LIANG Qiuyang
,
ZHONG Longbin
,
CHANG Huimin
,
CHEN Yunkun
,
QIAO Zhenshuan
. Study on multi-stage aqueous two-phase extraction of papain[J]. Food and Fermentation Industries, 2024
, 50(4)
: 152
-156
.
DOI: 10.13995/j.cnki.11-1802/ts.035787
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