To improve the added value of turtle products and develop new collagen raw materials, acid-soluble collagen (ASC) and enzyme-soluble collagen (ESC) were extracted from Chinese turtle powder by using citric acid, pepsin, acid protease, flavourzyme, and papain respectively. The amino acids, secondary structure, volatile substances, thermal stability, and other physicochemical parameters of the five collagens were determined. Results showed that all five collagens had the characteristics of type I collagen and were of high purity, but there were differences in the extraction efficiency between different methods. Among them, the pepsin-soluble collagen had a higher extraction rate and thermal stability and had the advantages of high subamino acid content, good protein cross-linking, and less odorous substances, which had good application prospects in the food and pharmaceutical fields. This study also provides a theoretical basis for the extraction and application of turtle-derived collagen.
HUANG Kecheng
,
BAO Jianqiang
,
ZHANG Xinggao
,
ZHENG Wen
,
ZHUANG Wenjing
,
GONG Xuan
. Comparison and physicochemical characterization of different extraction methods of collagen from Chinese soft-shelled turtle powder[J]. Food and Fermentation Industries, 2024
, 50(4)
: 204
-210
.
DOI: 10.13995/j.cnki.11-1802/ts.034411
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