Food and Fermentation Industries >

2024 , Vol. 50 >Issue 4: 302 - 307

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035912

Detection of diamide and dihydrazide pesticide residues in chicken by ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry

  • ZHANG Pengjie ,
  • LU Junwen ,
  • ZHANG Xianchen ,
  • LI Yong ,
  • HUANG Bijia ,
  • ZENG Yuanchang ,
  • LU Baiqi
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  • (Zhongshan Customs Technology Center, Zhongshan 528403, China)

Received date: 2023-04-22

  Revised date: 2023-06-13

  Online published: 2024-03-15

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Abstract

A method for the simultaneous determination of 8 diamides and 5 dihydrazide pesticide residues in chicken was established by ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry (UPLC-Q-Orbitrap HRMS). The sample was extracted with 1% acetate acetonitrile, salted out, and purified by the adsorbent, filtered by 0.22 μm membrane, and finally analyzed by UPLC-Q-Orbitrap HRMS. Gradient elution separation was carried out with 0.002 mol/L ammonium acetate solution and acetonitrile as mobile phases. The electrospray ionization was used and the positive and negative ions were scanned in the full scan and automatically triggered secondary mass spectrometry (Full MS/dd-MS2) mode. Quantification was performed by the chromatographic peak area of the parent ion, and qualitative confirmation was performed by the retention time and the secondary product ion mass spectrum obtained by data-dependent product ion scanning. The 13 pesticides showed good linear relationships within their respective linear ranges, with a correlation coefficient greater than 0.997, the average recoveries for blank samples at three different spiking levels were 90.1%-117.7%, with relative standard deviations from 1.2%-8.6%, with detection limits of 0.2-1.0 μg/kg. The method is simple, fast, and suitable for screening and confirmation of diamide and dihydrazide pesticide residues in chicken.

Key words： electrostatic field orbitrap high-resolution mass spectrometry; adsorbent purification; chicken; diamide pesticides; dihydrazide pesticides

Cite this article

ZHANG Pengjie , LU Junwen , ZHANG Xianchen , LI Yong , HUANG Bijia , ZENG Yuanchang , LU Baiqi . Detection of diamide and dihydrazide pesticide residues in chicken by ultra-performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry[J]. Food and Fermentation Industries, 2024 , 50(4) : 302 -307 . DOI: 10.13995/j.cnki.11-1802/ts.035912

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