The whole biological synthesis of glycolic acid has the characteristics of green and high efficiency. In the context of carbon neutralization, fully bio-based glycolate has received extensive attention, but its high fermentation cost and lack of downstream separation and purification technology have limited subsequent industrial applications. In this study, recombinant Escherichia coli was used as the object, the suitable glycolate production strains were screened by the high-throughput screening platform which constructed using the glycolate biosensor pGBS-PffS-sfgfp. The fermentation medium components were optimized by statistical method at the shaking flask level using cheap corncobs hydrolysate and other substrates, and preliminary amplification verification and downstream separation and purification were conducted based on 5 L fermenter. Finally, the optimal fermentation medium component was given as 6.66 mg/L CaCl2, 12 mg/L MgSO4·7H2O, 0.13 mg/L ZnCl2, 0.04 mg/L MnCl2·4H2O, 4.5 g/L KH2PO4, 13 g/L corncobs hydrolysate, 0.78 g/L NH3·H2O, 1.5 g/L yeast, 8.5 g/L tryptone, 0.5 g/L NaCl, 25.45 g/L Na2HPO4, the yield of glycolate reached 4.77 g/L, which was 2.58 times higher than the initial medium; the yield of 5 L tank was 42 g/L to 1.4 times before optimization. Preliminary biological separation and purification of glycolate fermentation solution was conducted by means of activated carbon decolorization, calcium hydroxide precipitation and cooling crystallization, and bio-based glycolate was successfully obtained, and the purity of glycolate was detected by HPLC. The results showed that the final glycolate crystal purity reached 99.3%, which met the commercial standard. In this study, through optimization of fermentation medium, amplification verification in 5 L fermentation tank, and downstream separation and purification, a low-cost production system of bio-based glycolate was initially constructed, which laid the foundation for industrial production of bio-based glycolate.
HU Chengjie
,
MAO Yin
,
LI Guohui
,
DENG Yu
. Production of glycolic acid from cheap biomass by Escherichia coli fermentation and its purification[J]. Food and Fermentation Industries, 2024
, 50(3)
: 21
-29
.
DOI: 10.13995/j.cnki.11-1802/ts.034939
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