Food and Fermentation Industries >

2024 , Vol. 50 >Issue 3: 38 - 44

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035424

Metabolic pathway and fermentation optimization of the biosynthesis of adipic acid

  • ZHI Rui ,
  • LI Guohui ,
  • MAO Yin ,
  • DENG Yu
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  • 1(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China)
    2(School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2023-03-09

  Revised date: 2023-03-31

  Online published: 2024-03-15

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Abstract

Employing the reverse adipate degradation pathway to synthesize adipic acid draws significant attention, but it's difficult to modify this metabolic pathway because of the long length and the imbalance heterologous expression of the pathway genes. To further increase the production of adipic acid, this study therefore modularized the reverse adipate degradation pathway, substituting the promoters of each module in order to regulate the expression profile of the pathway genes. By utilizing an adipic acid biosensor-based high-throughput screening method, the best combination resulted in a titer of 120.66 mg/L, which achieved an 8.35-fold improvement comparing to the control group. Moreover, the key enzymes of the pathway were confirmed through rational analysis and an efficiently regulation of the heterologous-expressed genes was eventually realized. Based on this, fermentation optimization was conducted and a titer of 240.49 mg/L adipic acid was eventually obtained, which was 16.67-fold higher than that of the control group. This study provided a new thought on the biosynthesis of adipic acid by modulating the pathway and regulating the gene expression profile.

Key words： adipic acid; constitutive expression; the reverse adipate degradation pathway; high-throughput screening; module modification

Cite this article

ZHI Rui , LI Guohui , MAO Yin , DENG Yu . Metabolic pathway and fermentation optimization of the biosynthesis of adipic acid[J]. Food and Fermentation Industries, 2024 , 50(3) : 38 -44 . DOI: 10.13995/j.cnki.11-1802/ts.035424

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