Food and Fermentation Industries >

2024 , Vol. 50 >Issue 3: 73 - 79

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.033892

Structural characterization of selenium-chondroitin sulfate nanoparticles and its effect on migration and invasion of Hela cells

  • CHEN Xuehua ,
  • CHEN Jianping ,
  • LUO Baozhen ,
  • LI Jiarui ,
  • LI Rui ,
  • LIU Xiaofei ,
  • SONG Bingbing ,
  • ZHONG Saiyi
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Laboratory for Marine Biological Products, Guangdong Province Engineering, Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Guangdong Provincial Modern Agricultural Science and Technology Innovation Center for Subtropical Fruit and Vegetable Processing, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing (Dalian Polytechnic University), Dalian 116034, China)

Received date: 2022-10-09

  Revised date: 2022-11-24

  Online published: 2024-03-15

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Abstract

This study aimed to identify the structure of selenium-chondroitin sulfate nanoparticles (SeCS) and investigate its effect on the migration and invasion of Hela cells. SeCS were prepared by the sodium selenite (Na2SeO3) and ascorbic acid redox reaction using chondroitin sulfate as a template and characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). The effects of SeCS on the migration and invasion of Hela cells were detected by scratch test and cell migration and invasion assay (Transwell). The effects of SeCS on matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression levels were detected using western blot. Results showed that the prepared SeCS were zero-valent spherical nanoparticles with a smooth surface. After Hela cells were treated with 20 μg/mL SeCS, the migration and invasion rates of cells were decreased from 100% in the control group to (59.19±7.74)% and (82.43±4.21)%, respectively, indicating that SeCS could inhibit cell migration and invasion. Further studies found that SeCS down-regulated the expression levels of MMP-2 and MMP-9 proteins, indicating that SeCS inhibited cell migration and invasion by reducing the expression of MMP-2 and MMP-9 proteins.

Key words： selenium-chondroitin sulfate nanoparticles; structural characterization; Hela cells; migration; invasion

Cite this article

CHEN Xuehua , CHEN Jianping , LUO Baozhen , LI Jiarui , LI Rui , LIU Xiaofei , SONG Bingbing , ZHONG Saiyi . Structural characterization of selenium-chondroitin sulfate nanoparticles and its effect on migration and invasion of Hela cells[J]. Food and Fermentation Industries, 2024 , 50(3) : 73 -79 . DOI: 10.13995/j.cnki.11-1802/ts.033892

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