As a unique species of chicken in China, the black-bone silky fowl has excellent haemopoietic properties. The black-bone silky fowl has been utilized to great advantage in the development of new blood supplement products. This study aimed to produce a sample of black-bone silky fowl peptide formula supplemented with the nutrients (EDTAFeNa, vitamins B1, B6, B12, and folic acid). The efficacy of the sample in restoring haematopoiesis was tested by feeding them to mice and the mechanism of haematopoiesis was investigated. The anemia model of mice was made by using cyclophosphamide and acetylphenylhydrazine, and the peripheral blood picture, bone marrow pathological observation, indirect bilirubin content, ATPase activity, hematopoietic factor level, and JAK2/STAT3 signaling pathway were detected. Results showed that black-bone silky fowl peptide nutrient formula could significantly restore peripheral blood cell and bone marrow haematopoietic stem cell populations. It reduced indirect bilirubin levels and increased ATPase activity, thus protecting red blood cells from rupture damage. It promoted hematopoietic cell recovery through the secretion of erythropoietin (EPO), human granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-3 (IL-3) hematopoietic factors, and regulated hematopoietic cell proliferation and differentiation through the JAK2/STAT3 signaling pathway. The study provided guidance for the development and utilization of black-bone silky fowl peptide.
ZHANG Zhuoran
,
LIAO Qun
,
FENG Zhiyuan
,
LIU Dan
,
LI Mingliang
,
LI Yiguang
,
REN Jie
,
XU Jinzhen
,
LIU Wenying
,
GU Ruizeng
,
LIU Wenjun
. Study on efficacy of black-bone silky fowl peptide nutrient formula based on JAK2/STAT3 signaling pathway in treatment of anemia[J]. Food and Fermentation Industries, 2024
, 50(3)
: 80
-85
.
DOI: 10.13995/j.cnki.11-1802/ts.033536
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