Effects of children growth milk powder on skeletal development in mice

  • YANG Li ,
  • LIU Tianxin ,
  • FANG Zhengfeng ,
  • AO Xiaolin ,
  • HAN Guoquan ,
  • CHEN Hong
Expand
  • (College of Food Science, Sichuan Agricultural University, Ya'an 625014, China)

Received date: 2022-10-21

  Revised date: 2022-11-23

  Online published: 2024-03-15

Abstract

This study aimed to compare the regulatory effects of two kinds of milk powder on skeletal dysplasia induced by dexamethasone in mice. Female ICR mice were randomly divided into a control group, dexamethasone group, dexamethasone + milk powder group 1, and dexamethasone + milk powder group 2, respectively. They were fed with corresponding diets for 8 weeks, and the skeletal dysplasia model was established by intramuscular injection of 1 mg/kg dexamethasone three times a week. At the end of the experiment, the changes in basic indexes, serum biochemical indexes, and intestinal flora were detected. Results showed that compared with the dexamethasone group, the two kinds of milk powder could improve the bone microstructure of mice. Serum biochemical analysis showed that milk powder supplementation increased serum bone turnover markers, decreased serum pro-inflammatory cytokines, and increased serum anti-inflammatory cytokines and serum calcium content. Intestinal microflora analysis showed that the composition of intestinal microflora could be changed after milk powder was supplemented. At the door level, the addition of milk powder 1 increased the relative abundance of Desulfobacterota, and the addition of milk powder 2 increased the relative abundance of Firmicutes but decreased the relative abundance of bacterioid OTA. At the genus level, the addition of milk powder 1 could increase the relative abundance of Akkermansia and Alloprevotella, while the addition of milk powder 2 could increase the relative abundance of Lactobacillus and Lachnoclostridium. To sum up, both kinds of milk powder can promote skeletal development, but there are differences in regulation mechanisms, which may be related to the unique composition of different milk powder formulas.

Cite this article

YANG Li , LIU Tianxin , FANG Zhengfeng , AO Xiaolin , HAN Guoquan , CHEN Hong . Effects of children growth milk powder on skeletal development in mice[J]. Food and Fermentation Industries, 2024 , 50(3) : 100 -109 . DOI: 10.13995/j.cnki.11-1802/ts.034059

References

[1] KUTTAPITIYA A, ASSI L, LAING K, et al.Microarray analysis of bone marrow lesions in osteoarthritis demonstrates upregulation of genes implicated in osteochondral turnover, neurogenesis and inflammation[J].Annals of the Rheumatic Diseases, 2017, 76(10):1764-1773.
[2] 吴南. 骨骼发育不良的遗传学研究进展[J].中华骨与关节外科杂志, 2021, 14(5):365-370.
WU N.Advances in the genetics of skeletal dysplasias[J].Chinese Journal of Bone and Joint Surgery, 2021, 14(5):365-370.
[3] ZHU B L, WANG X, LI L J.Human gut microbiome:The second genome of human body[J].Protein & Cell, 2010, 1(8):718-725.
[4] PACIFICI R.Role of gut microbiota in the skeletal response to PTH[J].The Journal of Clinical Endocrinology and Metabolism, 2021, 106(3):636-645.
[5] GUO D J, LIU W W, ZHANG X, et al.Duck egg white-derived peptide VSEE (val-ser-glu-glu) regulates bone and lipid metabolisms by Wnt/β-catenin signaling pathway and intestinal microbiota[J].Molecular Nutrition & Food Research, 2019,63(24):e1900525.
[6] TOMCZYK-WARUNEK A, DUBIEL A, POLESZAK E, et al.Disorders of the intestinal flora and it is effect on skeletal system diseases[J].Wiadomości Lekarskie, 2020, 73(9):1835-1839.
[7] WEAVER C M.Diet, gut microbiome, and bone health[J].Current Osteoporosis Reports, 2015, 13(2):125-130.
[8] RUBINSTEIN M R, WANG X W, LIU W, et al.Fusobacterium nucleatum promotes colorectal carcinogenesis by modulating E-cadherin/β-catenin signaling via its FadA adhesin[J].Cell Host & Microbe, 2013, 14(2):195-206.
[9] 汪晓娜. 日龄、钙和1, 25∶(OH)2∶D3对肉鸡生长性能、骨骼发育及小肠钙转运蛋白基因表达的影响[D].郑州:河南农业大学, 2021.
WANG X N.Effects of age, calcium and 1, 25-(OH)2-D3 on growth performance, bone development and small intestinal calcium transporter gene expression in broilers[D].Zhengzhou:Henan Agricultural University, 2021.
[10] SO D, WHELAN K, ROSSI M, et al.Dietary fiber intervention on gut microbiota composition in healthy adults:A systematic review and meta-analysis[J].The American Journal of Clinical Nutrition, 2018, 107(6):965-983.
[11] PATEL S, GOYAL A.Functional oligosaccharides:Production, properties and applications[J].World Journal of Microbiology and Biotechnology, 2011, 27(5):1119-1128.
[12] MARZIN P, CORMIER-DAIRE V.New perspectives on the treatment of skeletal dysplasia[J].Therapeutic Advances in Endocrinology and Metabolism,2020.DOI:10.1177/2042018820904016.
[13] MORTIER G R,COHN D H, CORMIER-DAIRE V, et al.Nosology and classification of genetic skeletal disorders:2019 revision[J].American Journal of Medical Genetics.Part A, 2019, 179(12):2393-2419.
[14] 王诗玮, 李玉姝.糖皮质激素诱导骨坏死[J].中华骨质疏松和骨矿盐疾病杂志, 2021, 14(4):404-411.
WANG S W, LI Y S.Glucocorticoid-induced osteonecrosis[J].Chinese Journal of Osteoporosis and Bone Mineral Research, 2021, 14(4):404-411.
[15] 张会丰, 王卫平, 杨毅, 等.地塞米松对大鼠骨骼纵向生长的直接抑制作用[J].中国儿童保健杂志, 2007, 15(5):500-501;504.
ZHANG H F, WANG W P, YANG Y, et al.Direct inhibition of dexamethasone on the longitudinal growth of bone in rats[J].Chinese Journal of Child Health Care, 2007, 15(5):500-501;504.
[16] 张会丰. 糖皮质激素抑制骨骼纵向生长机制的研究[D].上海:复旦大学, 2007.
ZHANG H F.Study on the mechanism of glucocorticoid inhibits the longitudinal growth of skeleton in rats[D].Shanghai:Fudan University, 2007.
[17] BONNET N, FERRARI S L.Effects of long-term supplementation with omega-3 fatty acids on longitudinal changes in bone mass and microstructure in mice[J].The Journal of Nutritional Biochemistry, 2011, 22(7):665-672.
[18] ROUSSEAU JAMES H, ALISON K, KENNY ANNE M.Self-reported dietary intake of omega-3 fatty acids and association with bone and lower extremity function[J].Journal of the American Geriatrics Society, 2009, 57(10):1781-1788.
[19] 谌红珊, 胡藩, 刘冬梅, 等.中老年人群骨密度与血钙、血磷、骨转换指标的关系[J].中国老年学杂志, 2017, 37(2):380-382.
CHEN H S, HU F, LIU D M, et al.Relationship between bone mineral density and blood calcium, blood phosphorus and bone turnover index in middle-aged and elderly people[J].Chinese Journal of Gerontology, 2017, 37(2):380-382.
[20] EICK G N, DEVLIN M J, CEPON-ROBINS T J, et al.A dried blood spot-based method to measure levels of tartrate-resistant acid phosphatase 5b (TRACP-5b), a marker of bone resorption[J].American Journal of Human Biology, 2019, 31(3):e23240.
[21] AI T Y, HAO L L, SHANG L C, et al.Konjac oligosaccharides modulate the gut environment and promote bone health in calcium-deficient mice[J].Journal of Agricultural and Food Chemistry, 2021, 69(15):4412-4422.
[22] CHEVALIER C, KIESER S, ÇOLAKOČLU M, et al.Warmth prevents bone loss through the gut microbiota[J].Cell Metabolism, 2020, 32(4):575-590.
[23] 成娟. 血清维生素D水平与PCOS患者胰岛素抵抗、骨代谢的相关性分析[J].中国医学工程, 2022, 30(2):40-43.
CHENG J.Correlation between serum vitamin D and insulin resistance, bone metabolism in PCOS patients[J].China Medical Engineering, 2022, 30(2):40-43.
[24] KIM M H, LEE G S, JUNG E M, et al.The negative effect of dexamethasone on calcium-processing gene expressions is associated with a glucocorticoid-induced calcium-absorbing disorder[J].Life Sciences, 2009, 85(3-4):146-152.
[25] LIU H R, LI D F, ZHANG Y, et al.Inflammation, mesenchymal stem cells and bone regeneration[J].Histochemistry and Cell Biology, 2018, 149(4):393-404.
[26] LEE J, BYUN H, MADHURAKKAT PERIKAMANA S K, et al.Current advances in immunomodulatory biomaterials for bone regeneration[J].Advanced Healthcare Materials, 2019, 8(4):e1801106.
[27] 钱刚. 磷脂酰丝氨酸在围术期神经认知障碍中作用的研究[D].济南:山东大学, 2020.
QIAN G.Study on the role of phosphatidylserine in perioperative neurocognitive impairment[D].Jinan:Shandong University, 2020.
[28] 白晓晔. 干酪乳杆菌Zhang和动物双歧杆菌乳亚种V9发酵乳缓解便秘机制研究[D].呼和浩特:内蒙古农业大学, 2020.
BAI X Y.Study on the mechanism of relieving constipation by fermented milk of Lactobacillus casei Zhang and Bifidobacterium animalis subsp.lactis V9[D].Hohhot:Inner Mongolia Agricultural University, 2020.
[29] 左玲玲. 乳酸菌发酵豆奶的蛋白结构表征及其致敏性的细胞学评估[D].南昌:南昌大学, 2019.
ZUO L L.Characterization of protein structure of soymilk fermented by lactic acid bacteria and cytological evaluation of its sensitization[D].Nanchang:Nanchang University, 2019.
[30] 杨茜, 李晓愚, 斯丹, 等.饮水中地塞米松污染对小鼠肠道菌群的影响[J].南方医科大学学报, 2016, 36(2):238-243.
YANG X, LI X Y, SI D, et al.Effect of dexamethasone contamination in drinking water on intestinal flora in mice[J].Journal of Southern Medical University, 2016, 36(2):238-243.
[31] BRUCE-KELLER A J, SALBAUM J M, LUO M, et al.Obese-type gut microbiota induce neurobehavioral changes in the absence of obesity[J].Biological Psychiatry, 2015, 77(7):607-615.
[32] LI C, HUANG Q, YANG R, et al.Gut microbiota composition and bone mineral loss—Epidemiologic evidence from individuals in Wuhan, China[J].Osteoporosis International, 2019, 30(5):1003-1013.
[33] TU M Y, HAN K Y, CHANG G R L, et al.Kefir peptides prevent estrogen deficiency-induced bone loss and modulate the structure of the gut microbiota in ovariectomized mice[J].Nutrients, 2020, 12(11):3432.
[34] WANG J H, WANG Y Y, GAO W J, et al.Diversity analysis of gut microbiota in osteoporosis and osteopenia patients[J].PeerJ, 2017, 5:e3450.
[35] ZHANG Y Q, LEE F Y, BARRERA G, et al.Activation of the nuclear receptor FXR improves hyperglycemia and hyperlipidemia in diabetic mice[J].Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(4):1006-1011.
[36] LIU J H, CHEN C Y, LIU Z Z, et al.Extracellular vesicles from child gut microbiota enter into bone to preserve bone mass and strength[J].Advanced Science, 2021, 8(9):2004831.
[37] WANG Z X, CHEN K, WU C C, et al.An emerging role of Prevotella histicola on estrogen deficiency-induced bone loss through the gut microbiota-bone axis in postmenopausal women and in ovariectomized mice[J].The American Journal of Clinical Nutrition, 2021, 114(4):1304-1313.
[38] ZHANG J, MOTYL K J, IRWIN R, et al.Loss of bone and Wnt10b expression in male type 1 diabetic mice is blocked by the probiotic Lactobacillus reuteri[J].Endocrinology, 2015, 156(9):3169-3182.
[39] LEMAIRE M, DOU S, CAHU A, et al.Addition of dairy lipids and probiotic Lactobacillus fermentum in infant formula programs gut microbiota and entero-insular axis in adult minipigs[J].Scientific Reports, 2018, 8:11656.
[40] YAN J, HERZOG J W, TSANG K, et al.Gut microbiota induce IGF-1 and promote bone formation and growth[J].Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(47):E7554-E7563.
[41] ARPAIA N, CAMPBELL C, FAN X Y, et al.Metabolites produced by commensal bacteria promote peripheral regulatory T-cell generation[J].Nature, 2013, 504(7480):451-455.
Outlines

/