Lactobacillus bulgaricus is an essential strain of yogurt starter and probiotic preparations.Its high-density culture has practical significance for improving production efficiency of the strain applied products.Glucose concentration and fermentation pH are key factors affecting the growth of Lactobacillus bulgaricus.The substrate glucose is converted into cellular bodies, and the main metabolite is lactic acid.In particular, the study of the rate of glucose consumption in relation to the rate of decrease in fermentation pH characterizing lactic acid production is an essential method to study the direction of metabolic flow in Lactobacillus bulgaricus.In this study, the effect of medium composition and culture conditions on the growth of Lactobacillus bulgaricus and the correlation model of glucose consumption rate-pH decrease rate was studied.Based on the conservation law of substrate mass, a feedback strategy for a high-density culture of Lactobacillus bulgaricus based on the pH decrease rate was established.The pH of fermentation was controlled within the interval of 6.0-5.0 using the chemical neutralization method, and the glucose concentration was controlled within the interval of 8-12 g/L by feedback feeding based on the pH decrease rate.The number of live bacteria in the fermentation liquid reached 3.6×109 CFU/mL, 2.4 times that of batch culture.The application results of this strategy provide a reference for improving the industrial production efficiency of Lactobacillus bulgaricus.
CHEN Xiaocen
,
XU Yan
,
LIU Yanmin
,
LI Xiaojun
,
MU Xiaoqing
. Feedback feeding strategy for high-density culture of Lactobacillus bulgaricus NQ2508 based on pH decrease rate[J]. Food and Fermentation Industries, 2024
, 50(5)
: 7
-13
.
DOI: 10.13995/j.cnki.11-1802/ts.035581
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