In the microbial fermentation process to produce pyrroloquinoline quinone (PQQ), the fed-batch fermentation method is commonly employed.However, microbial growth is slow in the early fermentation stage, and PQQ production decreases rapidly in the late fermentation stage.As a result, the production efficiency of the entire PQQ fed-batch fermentation may be low.To improve the production efficiency of PQQ fermentation, a preliminary study was conducted on the single-stage continuous fermentation process of PQQ utilizing Hyphomicrobium sp..When the PQQ fed-batch fermentation reached maximum PQQ productivity, it was converted to a single-stage continuous fermentation process, and the continuous fermentation was studied by turbidostat culture method and chemostat culture method, respectively.Experimental results showed that turbidostat culture with a dilution rate of 0.008 7 h-1 was the optimal continuous fermentation method for PQQ.The concentration of bacteria in the fermentation broth could be kept stable, and the fermentation yield was higher in turbidostat culture.Compared with the fed-batch fermentation process, the PQQ productivity of the turbidostat culture process increased by 80.7%, and the conversion rate of methanol substrate increased by 36.5%, which has good prospects for industrial application.
ZHAO Zigang
,
JIN Mingyi
,
LIU Lu
,
ZHANG Kui
. Preliminary study on continuous fermentation process of pyrroloquinoline quinone[J]. Food and Fermentation Industries, 2024
, 50(5)
: 29
-34
.
DOI: 10.13995/j.cnki.11-1802/ts.035799
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