Food and Fermentation Industries >

2024 , Vol. 50 >Issue 5: 131 - 141

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034421

Preparation of urolithin A liposomes:Stability and in vitro digestion studies

  • HU Yue ,
  • ZHANG Lu ,
  • WANG Siyu ,
  • WEI Linfeng ,
  • LU Feiyan ,
  • ZENG Peiyao ,
  • ZOU Liqiang ,
  • WEN Qinghui ,
  • TU Zongcai
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  • 1(National R&D Center for Freshwater Fish Processing, College of Life Science, Jiangxi Normal University, Nanchang 330022, China)
    2(Jiangxi Deshang Pharmaceutical Research Co.Ltd., Ganzhou 331200, China)
    3(State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330022, China)

Received date: 2022-11-24

  Revised date: 2023-01-10

  Online published: 2024-04-09

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Abstract

Urolithin A has many excellent physiological activities, but the extremely low water solubility and bioavailability limit its application.To overcome above limitations, the pH-driven method combined with high-pressure homogenization technology was used to prepare urolithin A liposomes (UA-LPs), and the structure, stability, and digestion characteristics of UA-LPs were investigated.Results showed that the average particle size of UA-LPs prepared by 20 mg/mL soybean lecithin was (97.46±0.83) nm, the polydispersity index, zeta potential, embedding rate and load rate was (0.27±0.01), (-40.3±1.06) mV, (98.11±0.26)%, and (2.39±0.01)%, respectively. UA-LPs had a spherical structure with uniform distribution under atomic force microscope.Thermal stability experiments showed that the embedding rate of UA-LPs with different wall material concentrations decreased with the extension of heating time, and UA-LPs prepared with 20 mg/mL soybean lecithin had the best thermal stability, and 45% of urolithin A were retained after 180 min treatment at 80 ℃, and the particle size and polydispersity coefficient changed little.The pH stability assays indicated a low embedding rate under acid condition, but the particle size and polydispersity coefficient did not change significantly with the increase of pH (P>0.05).The absolute value of zeta potential of UA-LPs prepared with 20 mg/mL soybean lecithin increased by 5.5, suggesting an increased stability.In vitro simulated digestion showed that UA-LPs could effectively improve the conversion rate and bio-acceptability of urolithin A, which increased by 3.26 and 2.07 times individually for that prepared with 20 mg/mL soybean lecithin compared with free urolithin A.Therefore, UA-LPs can be successfully prepared by pH driving method, and the physical stability of UA-LPs with high wall concentration is better.Above results can expand the application of urolithin A in the food and biomedicine industry.

Key words： urolithin A; liposomes; pH-driven method; stability; simulated digestion in vitro

Cite this article

HU Yue , ZHANG Lu , WANG Siyu , WEI Linfeng , LU Feiyan , ZENG Peiyao , ZOU Liqiang , WEN Qinghui , TU Zongcai . Preparation of urolithin A liposomes:Stability and in vitro digestion studies[J]. Food and Fermentation Industries, 2024 , 50(5) : 131 -141 . DOI: 10.13995/j.cnki.11-1802/ts.034421

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