The dry corned beef polypeptides were extracted by hydrochloric acid extraction method.Four kinds of antioxidant peptides were isolated and purified by AB-8 macroporous resin and C18 spherical silica gel column.The binding sites of four kinds of antioxidant peptides and superoxide dismutase (SOD) were simulated by molecular docking.The antioxidant peptides were extracted by hydrochloric acid extraction method and their antioxidant capacity was tested at 1, 2, 3, 4, and 5 mg/mL.The scavenging rates of DPPH free radicals, ABTS cationic radicals, and superoxide anion radicals were 86.24%, 35.62%, and 54.34%, respectively, at 5 mg/mL.The adsorption rate and desorption rate of AB-8, D101, S-8, X-S, HPD-500, and NKA-9 were compared.The adsorption rate and desorption rate of AB-8 were the best, which were 92.55% and 82.88%, respectively.The loading velocity, loading volume, ethanol elution concentration, and elution velocity of AB-8 were optimized, which were 4 BV/h of loading velocity, 39 mL of loading velocity, 75% (volume fraction) of ethanol solution elution agent, and 1 mL/min of elution velocity, respectively.Three components (A, B, and C) could be separated to test the antioxidant capacity of the three components.The scavenging capacity of DPPH free radicals, ABTS cationic radicals, and superoxide anion radicals of component B was the highest, which were 73.33%, 21.01%, and 63.33%, respectively.Four antioxidant peptides were isolated by C18 column separation of component B, including FDGDF, TGPGPW, FLSDH, and KPFDAK.Through molecular docking with SOD (PDB ID;1E9O), it was found that FDGDF, TGPGPW, FLSDH, and KPFDAK could form hydrogen bonds with SOD, and the binding energies were -6.7, -6.55, -6.6, and -7.35 kcal/mol, respectively.1 mg/mL of FDGDF could effectively increase SOD activity by 11.64%, and enzyme activity unit by 697 U/mL.It provides the theoretical basis for the development of dry corned beef products.
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