Phenylethanoid glycosides are particularly intriguing due to their physiological properties as an antioxidant and an anticancer.Many glycoside compounds can be prepared using glycosyltransferases as biocatalysts.In this study, UDP glycosyltransferase candidate genes were screened from the plant transcriptome of Penstemon barbatus, and their functions were determined to enrich the glycoside variety of phenylethanoid glycosides.Seven candidate genes were chosen for this study after being analyzed for differential gene expression in various plant tissues.They were then cloned into a pET-MBP expression vector, put into Escherichia coli Transetta (DE3) for heterologous expression, and their biological catalytic activity was tested using in vitro enzyme catalysis.It was discovered that a novel glycosyltransferase, UGT712A2, could catalyze the glucosylation of osmanthuside B C4-OH to form the new phenylethanoid glycoside, osmanthuside B-4-glucoside.UGT712A2 has a substrate breadth and can also catalyze the glycosylation of substances including verbascoside, rutin, picroside II, and 7-demethylsuberosin.In light of this, UGT712A2 may be a useful enzyme for producing phenylethanoid glycosides as well as a number of other novel glycoside products, opening the door to the identification of new glycoside molecules with potential for use in both food and medicine.
WU Yanan
,
YANG Yihan
,
DU Liping
,
ZHUANG Yibin
,
LIU Tao
. Screening and identification of new glycosyltransferases from Penstemon barbatus[J]. Food and Fermentation Industries, 2024
, 50(6)
: 17
-23
.
DOI: 10.13995/j.cnki.11-1802/ts.035776
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