Food and Fermentation Industries >

2024 , Vol. 50 >Issue 6: 40 - 46

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036103

Removal of bacterial biofilm from stainless steel surfaces by NaClO in combination with enzymes

  • HUANG Yangyang ,
  • DONG Zijie ,
  • WANG Shuli ,
  • CHAO Yuchao ,
  • LIU Chang ,
  • HUANG Zhongmin ,
  • SUO Biao ,
  • FAN Tao
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  • 1(College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China)
    2(Key Laboratory of Staple Grain Processing, Ministry of Agriculture and Rural Affairs, Zhengzhou 450002, China)
    3(National R&D Center For Frozen Rice& Wheat Produces Processing Technology, Zhengzhou 450002, China)
    4(Henan Grain Science Research Institute Co. Ltd., Zhengzhou, 450000, China)

Received date: 2023-05-10

  Revised date: 2023-05-19

  Online published: 2024-04-17

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Abstract

Bacterial biofilms colonizing different locations of equipment are the biggest challenge for the application of cleaning and disinfection procedures in the food industry.The purpose of this study was to screen the biofilm forming ability of different strains of bacteria by artificially culturing biofilms, using enzymes, NaClO, and a combination of both to remove mature films from stainless steel test materials, and to investigate the differences between the different treatments by means of plate counting and flow cytometry.Results showed that the anti-biofilm efficacy of enzymes varied depending on the stage of biofilm formation, with enzyme treatment tending to be more effective during biofilm formation than for mature biofilms, and partial enzyme involvement leading to the production of more biofilm substrates by bacteria.Bacterial counts showing that the three treatments resulted in reductions of 0.64 lg CFU/cm2 to 3.08 lg CFU/cm2 and 0.81 lg CFU/cm2 to 2.19 lg CFU/cm2 in Bacillus subtilis CICC 10900, Staphylococcus aureus ATCC 6538 cell populations, respectively. Flow cytometry results showed that 7.09%-12.04%, 22.43%-23.45%, and 31.33%-37.78% of the cells were damaged or died after treatment.The use of enzymes can help secondary agents such as NaClO to penetrate the biofilm more effectively and enhance the efficacy of removal of bacterial cells.The results of this study can provide data support and theoretical reference for the food industry when implementing biofilm removal procedures.

Key words： biofilms; stainless steel; enzyme; NaClO; flow cytometry

Cite this article

HUANG Yangyang , DONG Zijie , WANG Shuli , CHAO Yuchao , LIU Chang , HUANG Zhongmin , SUO Biao , FAN Tao . Removal of bacterial biofilm from stainless steel surfaces by NaClO in combination with enzymes[J]. Food and Fermentation Industries, 2024 , 50(6) : 40 -46 . DOI: 10.13995/j.cnki.11-1802/ts.036103

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