As people′s living standards improved gradually, the incidence of obesity is increasing rapidly.Lactobacillus is an excellent probiotic library and it has been found to have a preventive and therapeutic effect on obesity, and bile salt hydrolase (BSH) may play a key role.The specific degradation of bile salts by BSH makes the exploration and analysis of BSH from different strains particularly important.In this study, a strain of Ligilactobacillus sp.BD7642 with high BSH activity was found.This study explored the BSH from this strain. Ligilactobacillus sp.BD7642 with high BSH activity was screened by taurodeoxycholic acid sodium plate method.HPLC analyzed that Ligilactobacillus sp.BD7642 had a preference for taurocholic acid among 6 kinds of bile salts.Genome analysis of Ligilactobacillus sp.BD7642 revealed 3 genes (g1210, g1294, g1443) annotated as related to BSH, these 3 genes were heterologously expressed in E.coli BL21(DE3) and all of three expressed proteins had BSH activity, with g1294 having the highest BSH activity. Molecular docking showed that g1294 had a tighter binding site for taurine than other proteins, which interpreted its high enzyme activity and also provided new ideas and materials for future synthetic biology and artificial modification of strains.
[1] 范晓婧. 改革开放后中国民众的饮食生活[J].湘潮(下半月), 2016(2):78-82.
FAN X J.The dietetic life of Chinese people after the reform and opening up[J].Xiang Chao, 2016(2):78-82.
[2] SAKLAYEN M G.The global epidemic of the metabolic syndrome[J].Current Hypertension Reports, 2018, 20(2):12.
[3] ALLISON D B, FONTAINE K R, MANSON J E, et al.Annual deaths attributable to obesity in the United States[J].JAMA-Journal of the American Medical Association, 1999, 282(16):1530-1538.
[4] RENDELL M S.Obesity and diabetes:The final frontier[J].Expert Review of Endocrinology & Metabolism, 2023, 18(1):81-94.
[5] MOLINA-TIJERAS J A, DIEZ-ECHAVE P, VEZZA T, et al.Lactobacillus fermentum CECT5716 ameliorates high fat diet-induced obesity in mice through modulation of gut microbiota dysbiosis[J].Pharmacological Research, 2021, 167:105471.
[6] SUN Y N, CHEN S B, REN F Z, et al.Lactobacillus paracasei N1115 attenuates obesity in high-fat diet-induced obese mice[J].Food Science & Nutrition, 2022, 11(1):418-427.
[7] MAKWANA S, PRAJAPATI J B, PIPALIYA R, et al.Effects of probiotic fermented milk on management of obesity studied in high-fat-diet induced obese rat model[J].Food Production, Processing and Nutrition, 2023, 5(1):3.
[8] BOURGIN M, KRIAA A, MKAOUAR H, et al.Bile salt hydrolases:At the crossroads of microbiota and human health[J].Microorganisms, 2021, 9(6):1122.
[9] POLAND J C, FLYNN C R.Bile acids, their receptors, and the gut microbiota[J].Physiology, 2021, 36(4):235-245.
[10] RIDLON J M, KANG D J, HYLEMON P B, et al.Bile acids and the gut microbiome[J].Current Opinion in Gastroenterology, 2014, 30(3):332-338.
[11] ZHU H J, ZHAO F, ZHANG W J, et al.Cholesterol-lowering effect of bile salt hydrolase from a Lactobacillus johnsonii strain mediated by FXR pathway regulation[J].Food & Function, 2022, 13(2):725-736.
[12] LIANG C, ZHOU X H, GONG P M, et al.Lactiplantibacillus plantarum H-87 prevents high-fat diet-induced obesity by regulating bile acid metabolism in C57BL/6 J mice[J].Food & Function, 2021, 12(10):4315-4324.
[13] SONG Z W, CAI Y Y, LAO X Z, et al.Taxonomic profiling and populational patterns of bacterial bile salt hydrolase (BSH) genes based on worldwide human gut microbiome[J].Microbiome, 2019, 7(1):9.
[14] 夏永军, 王光强, 王巧惠, 等.HPLC分析植物乳杆菌胆盐水解酶降解特性[J].工业微生物, 2015, 45(6):16-20.
XIA Y J, WANG G Q, WANG Q H, et al.Degradation characteristics of BSH-1 from Lactobacillus plantarum by HPLC[J].Industrial Microbiology, 2015, 45(6):16-20.
[15] BI J, FANG F, LU S Y, et al.New insight into the catalytic properties of bile salt hydrolase[J].Journal of Molecular Catalysis B:Enzymatic, 2013, 96:46-51.
[16] WANG G Q, YU H I, FENG X, et al.Specific bile salt hydrolase genes in Lactobacillus plantarum AR113 and relationship with bile salt resistance[J].LWT, 2021, 145:111208.
[17] ŠUŠKOVIĆ J, KOS B, MATOŠIĆ S, et al.The effect of bile salts on survival and morphology of a potential probiotic strain Lactobacillus acidophilus M92[J].World Journal of Microbiology and Biotechnology, 2000, 16(7):673-678.
[18] CORZO G, GILLILAND S E.Measurement of bile salt hydrolase activity from Lactobacillus acidophilus based on disappearance of conjugated bile Salts1[J].Journal of Dairy Science, 1999, 82(3):466-471.
[19] BEGLEY M, HILL C, GAHAN C G M.Bile salt hydrolase activity in probiotics[J].Applied and Environmental Microbiology, 2006, 72(3):1729-1738.
[20] FOLEY M H, O’FLAHERTY S, ALLEN G, et al.Lactobacillus bile salt hydrolase substrate specificity governs bacterial fitness and host colonization[J].Proceedings of the National Academy of Sciences of the United States of America, 2021, 118(6):e2017709118.
[21] MCAULIFFE O, CANO R J, KLAENHAMMER T R.Genetic analysis of two bile salt hydrolase activities in Lactobacillus acidophilus NCFM[J].Applied and Environmental Microbiology, 2005, 71(8):4925-4929.
[22] GU X C, LUO X G, WANG C X, et al.Cloning and analysis of bile salt hydrolase genes from Lactobacillus plantarum CGMCC No.8198[J].Biotechnology Letters, 2014, 36(5):975-983.
[23] YANG J Y, ROY A, ZHANG Y.Protein-ligand binding site recognition using complementary binding-specific substructure comparison and sequence profile alignment[J].Bioinformatics, 2013, 29(20):2588-2595.
[24] WU Q, PENG Z L, ZHANG Y, et al. COACH-D:Improved protein-ligand binding sites prediction with refined ligand-binding poses through molecular docking[J].Nucleic Acids Research, 2018, 46(W1):W438-W442.
