Food and Fermentation Industries >

2024 , Vol. 50 >Issue 6: 84 - 91

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035678

Screening, mutagenesis breeding, and optimization of fermentation conditions of high-producing methionine endophytes

  • ZHU Qingyong ,
  • ZHANG Xin ,
  • DENG Ziling ,
  • Zhaxiduojie ,
  • YU Jiali ,
  • CHEN Qihe ,
  • LIU Zhengjie
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  • 1(College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, China)
    2(Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China)

Received date: 2023-03-30

  Revised date: 2023-04-30

  Online published: 2024-04-17

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Abstract

In this study, the endophytes in methionine-rich Rhus chinensis seeds were screened for high methionine producing strains.The methionine producing endophytes were screened, identified, and further bred with methionine analogs and 60Co-γ-ray mutagenesis.Subsequently, response surface methodology was used to investigate the effects of glucose, sodium sulfate and farnesol on methionine production of the strains.Results showed that the strain LB-2 with high methionine yield was identified as micrococcus by 16S rRNA sequence analysis and morphological identification.Subsequently, the strain LB-2-13 with higher methionine yield was obtained by mutagenesis breeding.The highest yield of methionine in the fermentation broth could be achieved at 2.85 g/L under the incubation conditions of 14.70 g/L glucose, 0.98 g/L sodium sulfate, and 96.35 μmol/L farnesol by single-factor and response surface method optimization, which increased the yield by about 88%.In this study, plant endophytes were used as the original strains to screen high methionine yielding strains for the first time.Furthermore, high-producing methionine strains were effectively bred with methionine analogs and 60Co γ-ray mutagenesis.This study could provide technical support for the industrial production of methionine by microbial fermentation.

Key words： micrococcus; methionine; endophytes; mutagenesis breeding; fermentation optimization

Cite this article

ZHU Qingyong , ZHANG Xin , DENG Ziling , Zhaxiduojie , YU Jiali , CHEN Qihe , LIU Zhengjie . Screening, mutagenesis breeding, and optimization of fermentation conditions of high-producing methionine endophytes[J]. Food and Fermentation Industries, 2024 , 50(6) : 84 -91 . DOI: 10.13995/j.cnki.11-1802/ts.035678

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