Antioxidant and antimicrobial properties of three monofloral kinds of honey from medicinal plants

  • ZHANG Guozhi ,
  • HAN Lingyun ,
  • LI Shanshan ,
  • ZHENG Huoqing ,
  • HU Fuliang
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  • (College of Animal Sciences, Zhejiang University, Hangzhou 310058, China)

Received date: 2022-12-21

  Revised date: 2023-02-07

  Online published: 2024-04-17

Abstract

The purpose of this study was to compare the antioxidant and antimicrobial activities of three monofloral kinds of honey from medicinal plants (fennel, wolfberry, and figwort) and to compare it with manuka honey.The antioxidant activities of honey samples were assessed by DPPH free radicals, ABTS cationic radicals scavenging activities, and ferric reducing/antioxidant power (FRAP) assay.The agar diffusion assay (zone of inhibition) and the broth micro-dilution assay were used to characterize honey samples against Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis), Gram-negative bacteria (Escherichia coli, Acinetobacter baumannii and Pseudomonas aeruginosa), and fungi (Candida albicans).Obtained results demonstrated that the total phenolic content and antioxidant activity of manuka honey were higher than those of the other three kinds of honey.There was a correlation between the antioxidant activity of honey and electrical conductivity, color value, and total phenolic content (r≥0.727, P<0.01).Fennel honey had the strongest antibacterial activity against S.aureus and S.epidermidis. The antibacterial activities of fennel honey and wolfberry honey against A.baumannii and P.aeruginosa were higher than that of manuka honey.The antibacterial activity of manuka honey against E.coli was higher than fennel honey, wolfberry honey, and figwort honey.The antibacterial activities of the three monofloral kinds of honey from medicinal plants were linearly correlated with pH, diastase activity, and hydrogen peroxide content.

Cite this article

ZHANG Guozhi , HAN Lingyun , LI Shanshan , ZHENG Huoqing , HU Fuliang . Antioxidant and antimicrobial properties of three monofloral kinds of honey from medicinal plants[J]. Food and Fermentation Industries, 2024 , 50(6) : 100 -108 . DOI: 10.13995/j.cnki.11-1802/ts.034676

References

[1] CIANCIOSI D, FORBES-HERNÁNDEZ T Y, AFRIN S, et al.Phenolic compounds in honey and their associated health benefits:A review[J].Molecules, 2018, 23(9):2322.
[2] SERAGLIO S K T, SILVA B, BERGAMO G, et al.An overview of physicochemical characteristics and health-promoting properties of honeydew honey[J].Food Research International, 2019, 119:44-66.
[3] ESCUREDO O, MÍGUEZ M, FERNÁNDEZ-GONZÁLEZ M, et al.Nutritional value and antioxidant activity of honeys produced in a European Atlantic area[J].Food Chemistry, 2013, 138(2-3):851-856.
[4] RANNEH Y, AKIM A M, HAMID H A, et al.Honey and its nutritional and anti-inflammatory value[J].BMC Complementary Medicine and Therapies, 2021, 21(1):30.
[5] ALVAREZ-SUAREZ J M, GASPARRINI M, FORBES-HERNÁNDEZ T Y, et al.The composition and biological activity of honey:A focus on Manuka honey[J].Foods, 2014, 3(3):420-432.
[6] GKOUTZOUVELIDOU M, PANOS G, XANTHOU M N, et al.Comparing the antimicrobial actions of Greek honeys from the island of Lemnos and Manuka honey from New Zealand against clinically important bacteria[J].Foods, 2021, 10(6):1042.
[7] PENTOŚ K, ŁUCZYCKA D, OSZMIAŃSKI J, et al.Polish honey as a source of antioxidants—A comparison with Manuka honey[J].Journal of Apicultural Research, 2020, 59(5):939-945.
[8] OTMANI A, AMESSIS-OUCHEMOUKH N, BIRINCI C, et al.Phenolic compounds and antioxidant and antibacterial activities of Algerian honeys[J].Food Bioscience, 2021, 42:101070.
[9] GOŚLIŃSKI M, NOWAK D, KŁĘBUKOWSKA L.Antioxidant properties and antimicrobial activity of Manuka honey versus Polish honeys[J].Journal of Food Science and Technology, 2020, 57(4):1269-1277.
[10] HULEA A, OBIŞTIOIU D, COCAN I, et al.Diversity of monofloral honey based on the antimicrobial and antioxidant potential[J].Antibiotics, 2022, 11(5):595.
[11] ANAND S, DEIGHTON M, LIVANOS G, et al.Agastache honey has superior antifungal activity in comparison with important commercial honeys[J].Scientific Reports, 2019, 9(1):18197.
[12] BUENO-COSTA F M, ZAMBIAZI R C, BOHMER B W, et al.Antibacterial and antioxidant activity of honeys from the state of Rio Grande do Sul, Brazil[J].LWT-Food Science and Technology, 2016, 65:333-340.
[13] MAJTAN J, BUCEKOVA M, KAFANTARIS I, et al.Honey antibacterial activity:A neglected aspect of honey quality assurance as functional food[J].Trends in Food Science & Technology, 2021, 118:870-886.
[14] DENG J L, LIU R, LU Q, et al.Biochemical properties, antibacterial and cellular antioxidant activities of buckwheat honey in comparison to Manuka honey[J].Food Chemistry, 2018, 252:243-249.
[15] ZHANG Y Z, SI J J, LI S S, et al.Chemical analyses and antimicrobial activity of nine kinds of unifloral Chinese honeys compared to Manuka honey (12+ and 20+)[J].Molecules, 2021, 26(9):2778.
[16] KUŚ P M, SZWEDA P, JERKOVIĆ I, et al.Activity of Polish unifloral honeys against pathogenic bacteria and its correlation with colour, phenolic content, antioxidant capacity and other parameters[J].Letters in Applied Microbiology, 2016, 62(3):269-276.
[17] ALVAREZ-SUAREZ J M, TULIPANI S, ROMANDINI S, et al.Contribution of honey in nutrition and human health:A review[J].Mediterranean Journal of Nutrition and Metabolism, 2010, 3(1):15-23.
[18] WANG K, WAN Z R, OU A Q, et al.Monofloral honey from a medical plant, Prunella vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats[J].Food & Function, 2019, 10(7):3828-3838.
[19] BOBIS O, MOISE A R, BALLESTEROS I, et al.Eucalyptus honey:Quality parameters, chemical composition and health-promoting properties[J].Food Chemistry, 2020, 325:126870.
[20] LIN T X, HUANG L, CHENG N N, et al.The in vitro and in vivo antibacterial activities of uniflorous honey from a medicinal plant, Scrophularia ningpoensis Hemsl., and characterization of its chemical profile with UPLC-MS/MS.[J].Journal of Ethnopharmacology, 2022, 296:115499.
[21] KADRI S M, ZALUSKI R, PEREIRA LIMA G P, et al.Characterization of Coffea arabica monofloral honey from Espírito Santo, Brazil[J].Food Chemistry, 2016, 203:252-257.
[22] BELAY A, SOLOMON W K, BULTOSSA G, et al.Botanical origin, colour, granulation, and sensory properties of the Harenna forest honey, Bale, Ethiopia[J].Food Chemistry, 2015, 167:213-219.
[23] BENTABOL MANZANARES A, HERNÁNDEZ GARCÍA Z, RODRÍGUEZ GALDÓN B, et al.Physicochemical characteristics of minor monofloral honeys from Tenerife, Spain[J].LWT-Food Science and Technology, 2014, 55(2):572-578.
[24] WHITE J W, COLLABORATORS , BEATY M R, et al. Instrumental color classification of honey: Collaborative study[J]. Journal of AOAC INTERNATIONAL, 1984, 67(6):1129-1131.
[25] ZHANG Y, WANG Y X, ZHAO H A, et al.Characterization of novel protein component as marker for floral origin of jujube (Ziziphus jujuba Mill.) honey[J].Journal of Agricultural and Food Chemistry, 2019, 67(44):12255-12263.
[26] WILCZYŃSKA A.Effect of filtration on colour, antioxidant activity and total phenolics of honey[J].LWT-Food Science and Technology, 2014, 57(2):767-774.
[27] BICUDO DE ALMEIDA-MURADIAN L, MONIKA BARTH O, DIETEMANN V, et al.Standard methods for Apis mellifera honey research[J].Journal of Apicultural Research, 2020, 59(3):1-62.
[28] GUO N N, ZHAO L W, ZHAO Y Z, et al.Comparison of the chemical composition and biological activity of mature and immature honey:An HPLC/QTOF/MS-based metabolomic approach[J].Journal of Agricultural and Food Chemistry, 2020, 68(13):4062-4071.
[29] CORVUCCI F, NOBILI L, MELUCCI D, et al.The discrimination of honey origin using melissopalynology and Raman spectroscopy techniques coupled with multivariate analysis[J].Food Chemistry, 2015, 169:297-304.
[30] MOHAMMED M E A.Factors affecting the physicochemical properties and chemical composition of bee′s honey[J].Food Reviews International, 2022, 38(6):1330-1341.
[31] EUROPEAN COUNCIL.Council directive 2001/110/EC of 20 December 2001 relating honey[J].Official Journal of the European Communities, 2002, 45: 47-52.
[32] CODEX ALIMENTARIUS COMMISSION.Revised codex standard for honey[S].Codex Standard, 2001, 12:1982.
[33] ALJOHAR H I, MAHER H M, ALBAQAMI J, et al.Physical and chemical screening of honey samples available in the Saudi market:An important aspect in the authentication process and quality assessment[J].Saudi Pharmaceutical Journal, 2018, 26(7):932-942.
[34] BOGDANOV S.Harmonised methods of the international honey commission[EB/OL].International Honey Commission, 2009.Retrieved from http://www.ihc-platform.net/ihcmethods2009.pdf.
[35] DA SILVA P M, GAUCHE C, GONZAGA L V, et al.Honey:Chemical composition, stability and authenticity[J].Food Chemistry, 2016, 196:309-323.
[36] BOUSSAID A, CHOUAIBI M, REZIG L, et al.Physicochemical and bioactive properties of six honey samples from various floral origins from Tunisia[J].Arabian Journal of Chemistry, 2018, 11(2):265-274.
[37] WON S R, LI C Y, KIM J W, et al.Immunological characterization of honey major protein and its application[J].Food Chemistry, 2009, 113(4):1334-1338.
[38] BRUDZYNSKI K, ABUBAKER K, ST-MARTIN L, et al.Re-examining the role of hydrogen peroxide in bacteriostatic and bactericidal activities of honey[J].Frontiers in Microbiology, 2011, 2:213.
[39] BUCEKOVA M, BUGAROVA V, GODOCIKOVA J, et al.Demanding new honey qualitative standard based on antibacterial activity[J].Foods, 2020, 9(9):1263.
[40] MAJTAN J, KLAUDINY J, BOHOVA J, et al.Methylglyoxal-induced modifications of significant honeybee proteinous components in Manuka honey:Possible therapeutic implications[J].Fitoterapia, 2012, 83(4):671-677.
[41] MAJTAN J, BOHOVA J, PROCHAZKA E, et al.Methylglyoxal may affect hydrogen peroxide accumulation in Manuka honey through the inhibition of glucose oxidase[J].Journal of Medicinal Food, 2014, 17(2):290-293.
[42] BRUDZYNSKI K.A current perspective on hydrogen peroxide production in honey.A review[J].Food Chemistry, 2020, 332:127229.
[43] ALZAHRANI H A, ALSABEHI R, BOUKRAÂ L, et al.Antibacterial and antioxidant potency of floral honeys from different botanical and geographical origins[J].Molecules, 2012, 17(9):10540-10549.
[44] 何亮亮, 赵浩安, 刘新艳, 等.枸杞蜜的抗氧化活性及其对DNA氧化损伤的保护作用[J].食品工业科技, 2019, 40(12):105-111.
HE L L, ZHAO H A, LIU X Y, et al.Antioxidant activity and protective effect on DNA oxidative damage of medlar honey[J].Science and Technology of Food Industry, 2019, 40(12):105-111.
[45] LACHMAN J, ORSÁK M, HEJTMÁNKOVÁ A, et al.Evaluation of antioxidant activity and total phenolics of selected Czech honeys[J].LWT-Food Science and Technology, 2010, 43(1):52-58.
[46] GHELDOF N, ENGESETH N J.Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples[J].Journal of Agricultural and Food Chemistry, 2002, 50(10):3050-3055.
[47] ZHANG G Z, TIAN J, ZHANG Y Z, et al.Investigation of the maturity evaluation indicator of honey in natural ripening process:The case of rape honey[J].Foods, 2021, 10(11):2882.
[48] TSAVEA E, VARDAKA F P, SAVVIDAKI E, et al.Physicochemical characterization and biological properties of pine honey produced across Greece[J].Foods, 2022, 11(7):943.
[49] HEMPATTARASUWAN P, SETTACHAIMONGKON S, DUANGMAL K.Impact of botanical source and processing conditions on physicochemical properties and antioxidant activity of honey in the northern part of Thailand[J].International Journal of Food Science & Technology, 2019, 54(12):3185-3195.
[50] KĘDZIERSKA-MATYSEK M, TETER A, STRYJECKA M, et al.Relationships linking the colour and elemental concentrations of blossom honeys with their antioxidant activity:A chemometric approach[J].Agriculture, 2021, 11(8):702.
[51] HALAGARDA M, GROTH S, POPEK S, et al.Antioxidant activity and phenolic profile of selected organic and conventional honeys from Poland[J].Antioxidants, 2020, 9(1):44.
[52] ANAND S, PANG E, LIVANOS G, et al.Characterization of physico-chemical properties and antioxidant capacities of bioactive honey produced from Australian grown Agastache rugosa and its correlation with colour and poly-phenol content[J].Molecules, 2018, 23(1):108.
[53] SAXENA S, GAUTAM S, SHARMA A.Physical, biochemical and antioxidant properties of some Indian honeys[J].Food Chemistry, 2010, 118(2):391-397.
[54] ALVAREZ-SUAREZ J M, TULIPANI S, DÍAZ D, et al.Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds[J].Food and Chemical Toxicology, 2010, 48(8-9):2490-2499.
[55] AVILA S, HORNUNG P S, TEIXEIRA G L, et al.Bioactive compounds and biological properties of Brazilian stingless bee honey have a strong relationship with the pollen floral origin[J].Food Research International, 2019, 123:1-10.
[56] GHELDOF N, WANG X H, ENGESETH N J.Identification and quantification of antioxidant components of honeys from various floral sources[J].Journal of Agricultural and Food Chemistry, 2002, 50(21):5870-5877.
[57] RAMÓN-SIERRA J M, VILLANUEVA M A, YAM-PUC A, et al.Antimicrobial and antioxidant activity of proteins isolated from Melipona beecheii honey[J].Food Chemistry-X, 2022, 13:100177.
[58] HOSSAIN M L, LIM L Y, HAMMER K, et al.A review of commonly used methodologies for assessing the antibacterial activity of honey and honey products[J].Antibiotics, 2022, 11(7):975.
[59] 王晶波, 秦文, 杨倬, 等.微孔板改良法评价多种国内外单花蜜的体外抑菌活性[J].中国食物与营养, 2019, 25(6):29-33.
WANG J B, QIN W, YANG Z, et al.Antibacterial activities of various domestic and foreign monofloral honeys in vitro by microplates modified method[J].Food and Nutrition in China, 2019, 25(6):29-33.
[60] ANTHIMIDOU E, MOSSIALOS D.Antibacterial activity of Greek and Cypriot honeys against Staphylococcus aureus and Pseudomonas aeruginosa in comparison to Manuka honey[J].Journal of Medicinal Food, 2013, 16(1):42-47.
[61] HERMANNS R, CREMERS N A J, LEEMING J P, et al.Sweet relief:Determining the antimicrobial activity of medical grade honey against vaginal isolates of Candida albicans[J].Journal of Fungi, 2019, 5(3):85.
[62] ALVAREZ-SUAREZ J M, GIAMPIERI F, BRENCIANI A, et al.Apis mellifera vs Melipona beecheii Cuban polifloral honeys:A comparison based on their physicochemical parameters, chemical composition and biological properties[J].LWT-Food Science and Technology, 2018, 87:272-279.
[63] JAVIER LEYVA-JIMENEZ F J, LOZANO-SANCHEZ J, BORRAS-LINARES I, et al.Potential antimicrobial activity of honey phenolic compounds against Gram positive and Gram negative bacteria[J].LWT-Food Science and Technology, 2019, 101:236-245.
[64] PELÁEZ-ACERO A, COBOS-VELASCO J E, GONZÁLEZ-LEMUS U, et al.Bioactive compounds and antibacterial activities in crystallized honey liquefied with ultrasound[J].Ultrasonics Sonochemistry, 2021, 76:105619.
[65] TRUCHADO P, LÓPEZ-GÁLVEZ F, GIL M I, et al.Quorum sensing inhibitory and antimicrobial activities of honeys and the relationship with individual phenolics[J].Food Chemistry, 2009, 115(4):1337-1344.
[66] ULUSOY E, KOLAYLI S, SARIKAYA A O.Antioxidant and antimicrobial activity of different floral origin honeys from Turkiye[J].Journal of Food Biochemistry, 2010, 34:321-335.
[67] FARKASOVSKA J, BUGAROVA V, GODOCIKOVA J, et al.The role of hydrogen peroxide in the antibacterial activity of different floral honeys[J].European Food Research and Technology, 2019, 245(12):2739-2744.
[68] BRUDZYNSKI K, SJAARDA C P.Colloidal structure of honey and its influence on antibacterial activity[J].Comprehensive Reviews in Food Science and Food Safety, 2021, 20(2):2063-2080.
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