Effect of oligosaccharides on antibacterial activity of Lactiplantibacillus plantarum AUH2103

  • YAN Haodong ,
  • ZHANG Chenchen ,
  • XU Jingjing ,
  • CHEN Dawei ,
  • KANG Wenli ,
  • PAN Lina ,
  • TANG Rongxue ,
  • LI Wei ,
  • GU Ruixia
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  • 1(College of Food Science and Engineering, Yangzhou University, Yangzhou 225000, China)
    2(Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control, Yangzhou 225000, China)
    3(Ausnutria Dairy Corporation Ltd., Changsha 410127, China)
    4(Hunan Engineering Research Center of Human Microecological Products, Changsha 410127, China)

Received date: 2023-03-29

  Revised date: 2023-04-23

  Online published: 2024-04-17

Abstract

The ability to inhibit pathogenic bacteria is an important indicator to evaluate the functional properties of probiotics.In this paper, the inhibition ability of Lactiplantibacillus plantarum AUH2103 was tested using a monolayer plate diffusion method and a cell adhesion method.The results showed that after replacing the carbon source with oligogalactose (GOS) and oligofructose (FOS), the inhibition ability of L.plantarum AUH2103 was not significantly different from the results in MRS;In contrast, the replacement of the carbon source with 2′-fucosyl lactose (2′-FL) resulted in insignificant inhibition due to weakened growth capacity.The adhesion of L.plantarum AUH2103 to Caco-2 became stronger in the presence of oligosaccharides;In the presence of L.plantarum AUH2103 and oligosaccharides, the adhesion ability of pathogenic bacteria to Caco-2 appeared to be significantly reduced.This study shows that in the presence of oligosaccharides, L.plantarum AUH2103 can inhibit the adhesion of pathogenic bacteria to Caco-2 cells.

Cite this article

YAN Haodong , ZHANG Chenchen , XU Jingjing , CHEN Dawei , KANG Wenli , PAN Lina , TANG Rongxue , LI Wei , GU Ruixia . Effect of oligosaccharides on antibacterial activity of Lactiplantibacillus plantarum AUH2103[J]. Food and Fermentation Industries, 2024 , 50(6) : 116 -121 . DOI: 10.13995/j.cnki.11-1802/ts.035657

References

[1] ECKBURG P B, BIK E M, BERNSTEIN C N, et al.Diversity of the human intestinal microbial flora[J].Science, 2005, 308(5728):1635-1638.
[2] FURCI F, CAMINATI M, CRISAFULLI E, et al.The intriguing possibility of using probiotics in allergen-specific immunotherapy[J].World Allergy Organization Journal, 2023, 16(2):100751.
[3] HANEISHI Y, FURUYA Y, HASEGAWA M, et al.Inflammatory bowel diseases and gut microbiota[J].International Journal of Molecular Sciences, 2023, 24(4):3817.
[4] QI Y, WANG X F.The role of gut microbiota in high-fat-diet-induced diabetes:Lessons from animal models and humans[J].Nutrients, 2023, 15(4):922.
[5] KAMADA N, CHEN G Y, INOHARA N, et al. Control of pathogens and pathobionts by the gut microbiota[J].Nature Immunology, 2013, 14:685-690.
[6] PIEWNGAM P, ZHENG Y, NGUYEN T H, et al.Pathogen elimination by probiotic Bacillus via signalling interference[J].Nature, 2018, 562:532-537.
[7] GIBSON G R, HUTKINS R, SANDERS M E, et al.Expert consensus document:The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics[J].Nature Reviews Gastroenterology and Hepatology, 2017, 14(8):491-502.
[8] ROBERFROID M, GIBSON G R, HOYLES L, et al.Prebiotic effects:Metabolic and health benefits[J].The British Journal of Nutrition, 2010, 104(Suppl 2):1-63.
[9] GARRIDO D, RUIZ-MOYANO S, LEMAY D G, et al.Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria[J].Scientific Reports, 2015, 5:13517.
[10] OOZEER R, VAN LIMPT K, LUDWIG T, et al. Intestinal microbiology in early life: Specific prebiotics can have similar functionalities as human-milk oligosaccharides1[J]. The American Journal of Clinical Nutrition, 2013, 98(2):561S-571S.
[11] RUIZ-PALACIOS G M, CERVANTES L E, RAMOS P, et al.Campylobacter jejuni binds intestinal H(O) antigen (Fuc alpha 1, 2Gal beta 1, 4GlcNAc), and fucosyloligosaccharides of human milk inhibit its binding and infection[J].The Journal of Biological Chemistry, 2003, 278(16):14112-14120.
[12] YU Z T, NANTHAKUMAR N N, NEWBURG D S.The human milk oligosaccharide 2′-fucosyllactose quenches Campylobacter jejuni-induced inflammation in human epithelial cells HEp-2 and HT-29 and in mouse intestinal Mucosa1[J].The Journal of Nutrition, 2016, 146(10):1980-1990.
[13] BOHORA A A, KOKATE S R, KHEDKAR S, et al.Antimicrobial activity of probiotics against endodontic pathogens:A preliminary study[J].Indian Journal of Medical Microbiology, 2019, 37(1):5-11.
[14] ALIZADEH BEHBAHANI B, NOSHAD M, FALAH F.Inhibition of Escherichia coli adhesion to human intestinal Caco-2 cells by probiotic candidate Lactobacillus plantarum strain L15[J].Microbial Pathogenesis, 2019, 136:103677.
[15] SINGH T P, KAUR G, KAPILA S, et al.Antagonistic activity of Lactobacillus reuteri strains on the adhesion characteristics of selected pathogens[J].Frontiers in Microbiology, 2017, 8:486.
[16] 王鹏, 刚梦萱, 张臣臣, 等.母乳源乳酸菌的低聚糖利用特性研究[J].食品与发酵工业, 2022, 48(11):101-106.
WANG P, GANG M X, ZHANG C C, et al.Oligosaccharide utilization characteristics of lactic acid bacteria from breast milk[J].Food and Fermentation Industries, 2022, 48(11):101-106.
[17] COPPA G V, BRUNI S, ZAMPINI L, et al. Oligosaccharides of human milk inhibit the adhesion of listeria monocytogenes to Caco-2 cells[J].The Italian Journal of Pediatrics, 2003, 29(1):61-68.
[18] ELO S, SAXELIN M, SALMINEN S. Attachment of Lactobacillus casei strain GG to human colon carcinoma cell line Caco-2:Comparison with other dairy strains Lett[J].Letters in Applied Microbiology, 1991, 13(3):154-156.
[19] CHAUVIERE G, COCONNIER M H, KERNEIS S, et al.Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells[J].Journal of General Microbiology, 1992, 138(8):1689-1696.
[20] BERNET M F, BRASSART D, NEESER J R, et al.Adhesion of human bifidobacterial strains to cultured human intestinal epithelial cells and inhibition of enteropathogen-cell interactions[J].Applied and Environmental Microbiology, 1993,59(12):4121-4128.
[21] BEACHEY E H.Bacterial adherence:Adhesin-receptor interactions mediating the attachment of bacteria to mucosal surfaces[J].The Journal of Infectious Diseases, 1981, 143(3):325-345.
[22] GRIGORYAN S, BAZUKYAN I, TRCHOUNIAN A.Aggregation and adhesion activity of lactobacilli isolated from fermented products in vitro and in vivo:A potential probiotic strain[J].Probiotics and Antimicrobial Proteins, 2018, 10(2):269-276.
[23] ENDO H, TAMURA K, FUKASAWA T, et al.Comparison of fructooligosaccharide utilization by Lactobacillus and Bacteroides species[J].Bioscience, Biotechnology, and Biochemistry, 2012, 76(1):176-179.
[24] FACINELLI B, MARINI E, MAGI G, et al.Breast milk oligosaccharides:Effects of 2′-fucosyllactose and 6′-sialyllactose on the adhesion of Escherichia coli and Salmonella fyris to Caco-2 cells[J].The Journal of Maternal-Fetal and Neonatal Medicine, 2019, 32(17):2950-2952.
[25] CILIEBORG M S, SANGILD P T, JENSEN M L, et al.α1,2-Fucosyllactose does not improve intestinal function or prevent Escherichia coli F18 diarrhea in newborn pigs[J].Journal of Pediatric Gastroenterology and Nutrition, 2017, 64(2):310-318.
[26] JANKOWSKA A, LAUBITZ D, ANTUSHEVICH H, et al.Competition of Lactobacillus paracasei with Salmonella enterica for adhesion to Caco-2 cells[J].Journal of Biomedicine and Biotechnology, 2008, 2008:357964.
[27] JAYASHREE S, KARTHIKEYAN R, NITHYALAKSHMI S, et al.Anti-adhesion property of the potential probiotic strain Lactobacillus fermentum 8711 against methicillin-resistant Staphylococcus aureus (MRSA)[J].Frontiers in Microbiology, 2018, 9:411.
[28] COCONNIER M H, BERNET M F, KERNÉIS S, et al.Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion[J].FEMS Microbiology Letters, 1993, 110(3):299-305.
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