Food and Fermentation Industries >

2024 , Vol. 50 >Issue 6: 192 - 200

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.034740

Optimization of extraction of phenolics from Sargassum pallidum by response surface methodology and its antioxidant and antidiabetic activities

  • XIE Xing ,
  • WANG Lehuai ,
  • LIN Wenjing ,
  • LIU Xiaohuan ,
  • ZHANG Lu ,
  • TU Zongcai
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  • 1(College of Health, Jiangxi Normal University, Nanchang 330022, China)
    2(State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China)

Received date: 2022-12-28

  Revised date: 2023-01-31

  Online published: 2024-04-17

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Abstract

Sargassum pallidum is an edible and medicinal brown seaweed with various functional activities.In this study, the ultrasonic-assisted extraction technology of phenolics from Sargassum pallidum was optimized by using the response surface method.The total phenolics content, antioxidant, and antidiabetic activities of polyphenol extract in Sargassum pallidum and its fraction were also investigated.Results indicated that the optimum extraction process condition was the ethanol concentration of 8.5%, extraction time of 55 min, extraction temperature of 42 ℃, and ultrasonic power of 494 W, the phenolics content of Sargassum pallidum was 1.81 mg GAE/g DM.The n-butanol fraction of crude extract from Sargassum pallidum showed the highest phenolics content with a value of 24.45 mg GAE/g E.Biological studies displayed that dichloromethane fraction gave the strongest DPPH radical scavenging capacity (49.34 μmol TE/g) and ABTS cationic radical scavenging capacity (IC50=249.22 μg/mL).The α-glucosidase inhibitory of dichloromethane and ethyl acetate fractions was 10.33 and 9.61 times higher than that of acarbose.Meanwhile, the ethyl acetate fraction had the best DPP-Ⅳ enzyme inhibition activity with an IC50 value of 25.83 μg/mL, and the solvent showed a good enrichment effect on antidiabetic components in Sargassum pallidum.Correlative analysis suggested that phenolics were the major antioxidants in Sargassum pallidum, and the inhibition activities of α-glucosidase and DPP-Ⅳ enzyme were closely correlated.This study found that the phenolics in Sargassum pallidum showed great potential as multi-target drugs or health food, which also could promote the exploration of high value-added products from Sargassum pallidum.

Key words： Sargassum pallidum; phenolics; response surface methodology; antioxidation activity; antidiabetic activity

Cite this article

XIE Xing , WANG Lehuai , LIN Wenjing , LIU Xiaohuan , ZHANG Lu , TU Zongcai . Optimization of extraction of phenolics from Sargassum pallidum by response surface methodology and its antioxidant and antidiabetic activities[J]. Food and Fermentation Industries, 2024 , 50(6) : 192 -200 . DOI: 10.13995/j.cnki.11-1802/ts.034740

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