Dendrobine is the characteristic ingredient of the traditional plant Dendrobium nobile with medicinal activity, structurally belonging to the dendrobine-type total alkaloids (DTTAs).The accumulation of dendrobine is influenced by the cycle and the environment, and it is difficult to obtain from plants alone to meet the demand.Trichoderma longibrachiatum UN32, the subject of this study, is a DTTAs-producing positive mutant obtained by compound mutagenesis in the previous study. To improve the DTTAs yield of UN32 and stabilize the expression level of the product, this study determined the optimal ratio of metal ions from adding inorganic salts in the fermentation medium of UN32 using response surface methodology.The three factors that had the most significant effect on the response results were Zn2+, Cu2+and Fe2+, and the optimum addition times and concentrations of Cu2+, Fe2+, and Zn2+ were 0.5 mmol/L on the 6th day, 0.4 mmol/L on the 6th day and 0.7 mmol/L on the 3rd day, respectively.The results of the response surface statistics showed that the alkaloid production reached (317.36±6.48) μg when the model response value alkaloid amount reached its maximum at concentrations of 0.54, 0.69, and 0.39 mmol/L for Cu2+, Zn2+, and Fe2+, respectively, which was 72.68% higher than the initial medium.In summary, the metal ions and their different combinations have promoted the accumulation of alkaloids in strain UN32, and the method has good development value and application prospects.
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