Food and Fermentation Industries >

2024 , Vol. 50 >Issue 6: 267 - 273

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035750

Development and performance verification of a coliform test strip based on lactose bile salt system

  • WANG Jiewei ,
  • SUN Wandong ,
  • WU Yanhui ,
  • HE Yanling
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  • 1(Beijing Landbridge Technology Co.Ltd., Beijing 100123, China)
    2(Chinese Academy of Inspection and Quarantine, Beijing 100176, China)

Received date: 2023-04-07

  Revised date: 2023-05-15

  Online published: 2024-04-17

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Abstract

This study aimed to develop an accurate and rapid test strip for the detection of coliform bacteria in food samples, as a complementary solution to address the limitations of long detection cycles and low detection rates of weak-fermenting bacteria in national standard detection methods.Based on the composition of the violet red bile agar (VRBA), the coliform test strip was developed by screening different gelling agents, optimizing the concentration of gelling agent, lactose and bile salt using an orthogonal test, and including a lactose fermentation inducer.Results showed that the physicochemical and microbial properties of the coliform test strip reached the highest level when carrageenan was used instead of agar as the cold water soluble gel, carrageenan, lactose and bile salt were added at 15, 10, and 1.2 g/L, respectively, and lactyl-N fatty acyl sphingosine was added at 250 mg/L as the lactose fermentation inducer.The performance of the test strips met the control requirements for VRBA culture medium using the national standard method.Compared with the national standard plate count method for coliform bacteria, the test strip method reduced the detection time by 24 hours without significant differences in the actual sample detection results.In addition, result interpretation with the test strip was more intuitive and the detection ability for weakly gas-producing target bacteria was improved.The coliform test strip developed in this study is easy to use, fast and accurate.It is suitable for food testing and can serve as a good reference for the development of commercial test strips.

Key words： coliform; strip; cold water soluble gel; inducer

Cite this article

WANG Jiewei , SUN Wandong , WU Yanhui , HE Yanling . Development and performance verification of a coliform test strip based on lactose bile salt system[J]. Food and Fermentation Industries, 2024 , 50(6) : 267 -273 . DOI: 10.13995/j.cnki.11-1802/ts.035750

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