Food and Fermentation Industries >

2024 , Vol. 50 >Issue 7: 39 - 45

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038161

Anti-fatigue effects and mechanism of Ganoderma lucidum spore oil on mice

  • YU Mengqi ,
  • HUANG Shuzhen ,
  • LIU Yufei ,
  • YANG Hua ,
  • CAO Li ,
  • ZHANG Lishi ,
  • CHEN Jinyao
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  • 1(West China School of Public Health/West China Fourth Hospital, Sichuan University, Chengdu 610041, China)
    2(Key Laboratory of Food Safety Monitoring and Risk Assessment, Chengdu 610041, China)

Received date: 2023-12-04

  Revised date: 2023-12-13

  Online published: 2024-05-09

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Abstract

To evaluate the effect of Ganoderma lucidum spore oil (GLSO) on alleviating physical fatigue in mice and explore its potential mechanism.144 SPF-grade male KM mice were randomly divided into four groups:solvent control group, low-dose GLSO group, medium-dose GLSO group, and high-dose GLSO group, 36 mice in each group, administered orally for 30 days.Mice were divided into four batches, which were used for measuring weight-bearing swimming time (I), liver glycogen levels (II), blood lactic acid levels (IV), serum urea nitrogen levels, muscle glycogen levels, and other indicators (including gastrocnemius HE staining, PAS staining, and TUNEL staining) (III), respectively.Results showed that compared with the solvent control group, the weight-bearing swimming time of the mice in medium-dose GLSO group significantly prolonged (P<0.01);the serum urea nitrogen levels of mice in all dose groups of GLSO significantly reduced (P<0.01);the levels of liver glycogen and muscle glycogen in all groups showed no significant difference (P>0.05);the area under the curve of blood lactic acid of mice in all dose groups of GLSO significantly reduced (P<0.01).HE staining results showed no obvious pathological changes in each group.PAS staining results showed that the positive area of muscle glycogen in the high-dose GLSO group significantly increased (P<0.05).TUNEL staining results showed that the positive rate of apoptotic cells in the low-dose GLSO group significantly reduced (P<0.05).Thus, GLSO can relieve physical fatigue, and its mechanism may be related to reducing glycogen consumption and the apoptosis of gastrocnemius muscle cells.

Key words： Ganoderma lucidum spore oil; anti-fatigue effect; PAS staining; TUNEL staining; mice

Cite this article

YU Mengqi , HUANG Shuzhen , LIU Yufei , YANG Hua , CAO Li , ZHANG Lishi , CHEN Jinyao . Anti-fatigue effects and mechanism of Ganoderma lucidum spore oil on mice[J]. Food and Fermentation Industries, 2024 , 50(7) : 39 -45 . DOI: 10.13995/j.cnki.11-1802/ts.038161

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