This study aimed to study the protective effect of ginsenosides on oxidative stress injury of human colon cancer (Caco-2) cells induced by hydrogen peroxide.Human colon cancer Caco-2 cells were cultured to construct the oxidative damage model of Caco-2 cells induced by hydrogen peroxide.The oxidative stress model of Caco-2 cells treated by ginsenosides at different concentrations (10, 20, and 50 μg/mL) was used to observe the cell vitality by morphological observation and the CCK-8 method.The intracellular superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were determined.The activity of oxidative damage marker malondialdehyde (MDA) and reactive oxygen species (ROS) were measured to evaluate the antioxidant stress level of ginsenosides.Results showed that the survival rate of Caco-2 cells reached about 58% after inducing Caco-2 cells with 200 μmol/L H2O2 for 4 h, which met the modelling requirements.Ginsenosides (10-50 μg/mL) significantly increased the survival rate, SOD, and intracellular GSH-Px activity, and decreased the level of ROS and MDA in the damaged cells.Ginsenosides can protect Caco-2 cells from oxidative stress induced by hydrogen peroxide.
LIU Xiaofeng
,
LU Xiaoqin
,
ZHONG Hao
,
HUSSAIN Muhammad
,
WANG Lina
,
YANG Mengyu
,
ZHANG Jianing
,
GUAN Rongfa
. Protective effect of ginsenosides on hydrogen peroxide-induced oxidative damage of Caco-2 cells[J]. Food and Fermentation Industries, 2024
, 50(7)
: 46
-50
.
DOI: 10.13995/j.cnki.11-1802/ts.035145
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