Stable turmeric liposomes were prepared using high-pressure homogenization technology, followed by the combination with spray drying technology to produce high-load turmeric liposome powder.The physicochemical properties such as particle size, zeta potential, and microstructure were analyzed, along with an evaluation of storage stability and in vitro and in vivo absorption performance.Results showed that phospholipids played a filling role, making the microcapsules more regular and fuller in shape.When the phospholipid content was 10%, and curcumin content was 6%, the turmeric liposome powder had good storage stability.The turmeric liposome powder significantly improved the bioaccessibility of curcumin, which was approximately 11 times that of the turmeric raw material and 4 times that of the commercial high-absorption turmeric.Animal experimental results showed that the turmeric liposome powder significantly enhanced the oral absorption performance of curcumin.The area under curve (AUC) of total curcumin of turmeric liposome powder was 8.1 times that of the turmeric raw material and 4.13 times that of the commercial high-absorption turmeric.The liposome powder prepared using high-pressure homogenization and spray drying technology was a reliable technique for achieving high loading of curcumin, laying a theoretical foundation for the development of turmeric-related products.
[1] KUNNUMAKKARA A B, BORDOLOI D, PADMAVATHI G, et al.Curcumin, the golden nutraceutical:Multitargeting for multiple chronic diseases[J].British Journal of Pharmacology, 2017, 174(11):1325-1348.
[2] SOLEIMANI V, SAHEBKAR A, HOSSEINZADEH H.Turmeric (Curcuma longa) and its major constituent (curcumin) as nontoxic and safe substances:Review[J].Phytotherapy Research:PTR, 2018, 32(6):985-995.
[3] RAI M, PANDIT R, GAIKWAD S, et al.Potential applications of curcumin and curcumin nanoparticles:From traditional therapeutics to modern nanomedicine[J].Nanotechnology Reviews, 2015, 4(2):161-172.
[4] WANG Q R, YE C Q, SUN S K, et al.Curcumin attenuates collagen-induced rat arthritis via anti-inflammatory and apoptotic effects[J].International Immunopharmacology, 2019, 72:292-300.
[5] WANG L, MU R J, LI Y Z, et al.Characterization and antibacterial activity evaluation of curcumin loaded konjac glucomannan and zein nanofibril films[J].LWT, 2019, 113:108293.
[6] JIANG T, CHARCOSSET C.Encapsulation of curcumin within oil-in-water emulsions prepared by premix membrane emulsification:Impact of droplet size and carrier oil on the chemical stability of curcumin[J].Food Research International, 2022, 157:111475.
[7] SHAO W J, YU Z W, CHIANG Y T, et al.Curcumin prevents high fat diet induced insulin resistance and obesity via attenuating lipogenesis in liver and inflammatory pathway in adipocytes[J].PLoS One, 2012, 7(1):e28784.
[8] 刘佳, 黄宇虹, 王保和, 等.姜黄素类化合物体内代谢途径及其代谢产物的研究进展[J].现代药物与临床, 2015, 30(12):1553-1557.
LIU J, HUANG Y H, WANG B H, et al.Research progress on the metabolic pathways in vivo and their metabolites of curcuminoids[J].Drugs & Clinic, 2015, 30(12):1553-1557.
[9] HOEHLE S I, PFEIFFER E, SÓLYOM A M, et al.Metabolism of curcuminoids in tissue slices and subcellular fractions from rat liver[J].Journal of Agricultural and Food Chemistry, 2006, 54(3):756-764.
[10] HYUN J E, YI H Y, HONG G P, et al.Digestion stability of curcumin-loaded nanostructured lipid carrier[J].LWT, 2022, 162:113474.
[11] ESPOSTO B S, JAUREGI P, TAPIA-BLÁCIDO D R, et al.Liposomes vs.chitosomes:Encapsulating food bioactives[J].Trends in Food Science & Technology, 2021, 108:40-48.
[12] LIU W L, YE A Q, LIU W, et al.Stability during in vitro digestion of lactoferrin-loaded liposomes prepared from milk fat globule membrane-derived phospholipids[J].Journal of Dairy Science, 2013, 96(4):2061-2070.
[13] LIU H, ZHANG J B, XIONG Y, et al.Improving norbixin dispersibility and stability by liposomal encapsulation using the pH-driven method[J].Journal of the Science of Food and Agriculture, 2022, 102(5):2070-2079.
[14] PENG S F, ZOU L Q, LIU W, et al.Fabrication and characterization of curcumin-loaded liposomes formed from sunflower lecithin:Impact of composition and environmental stress[J].Journal of Agricultural and Food Chemistry, 2018, 66(46):12421-12430.
[15] PETRALITO S, SPERA R, PACELLI S, et al.Design and development of PEG-DMA gel-in-liposomes as a new tool for drug delivery[J].Reactive and Functional Polymers, 2014, 77:30-38.
[16] LAJAVARDI L, CAMELO S, AGNELY F, et al.New formulation of vasoactive intestinal peptide using liposomes in hyaluronic acid gel for uveitis[J].Journal of Controlled Release, 2009, 139(1):22-30.
[17] 黄笛. 高压均质结合酶法改性对蛋清液加工性能的影响[D].雅安:四川农业大学, 2022.
HUANG D.Effect of high pressure homogenization and enzymatic modification on the processing performance of egg white liquid[D].Ya’an:Sichuan Agricultural University, 2022.
[18] GAO H X, CHENG C, FANG S Q, et al.Study on curcumin encapsulated in whole nutritional food model milk:Effect of fat content, and partitioning situation[J].Journal of Functional Foods, 2022, 90:104990.
[19] PENG S F, LI Z L, ZOU L Q, et al.Enhancement of curcumin bioavailability by encapsulation in sophorolipid-coated nanoparticles:An in vitro and in vivo study[J].Journal of Agricultural and Food Chemistry, 2018, 66(6):1488-1497.
[20] XIE X X, TAO Q, ZOU Y N, et al.PLGA nanoparticles improve the oral bioavailability of curcumin in rats:Characterizations and mechanisms[J].Journal of Agricultural and Food Chemistry, 2011, 59(17):9280-9289.
[21] LI Y Y, SUN K Y, CHEN S N, et al.Nano-resveratrol liposome:Physicochemical stability, in vitro release, and cytotoxicity[J].Applied Biochemistry and Biotechnology, 2023, 195(10):5950-5965.
[22] 张转转. 褐藻胶寡糖纳米脂质体的制备及其生物学功能研究[D].杭州:中国计量大学, 2016.
ZHANG Z Z.Preparation and biological function of alginate oligosaccharide nanoliposomes[D].Hangzhou:China University of Metrology, 2016.
[23] SRIHERA N, LI Y, ZHANG T T, et al.Preparation and characterization of astaxanthin-loaded liposomes stabilized by sea cucumber sulfated sterols instead of cholesterol[J].Journal of Oleo Science, 2022, 71(3):401-410.
[24] XIE Y L, ZHOU H M, LIANG X H, et al.Study on the morphology, particle size and thermal properties of vitamin A microencapsulated by starch octenylsucciniate[J].Agricultural Sciences in China, 2010, 9(7):1058-1064.
[25] NEVES M I L, DESOBRY-BANON S, PERRONE I T, et al.Encapsulation of curcumin in milk powders by spray-drying:Physicochemistry, rehydration properties, and stability during storage[J].Powder Technology, 2019, 345:601-607.
[26] SILVA G S, JANGE C G, ROCHA J S S, et al.Characterisation of curcumin-loaded proliposomes produced by coating of micronised sucrose and hydration of phospholipid powders to obtain multilamellar liposomes[J].International Journal of Food Science & Technology, 2017, 52(3):772-780.
