To explore the potential of carboxymethyl citrus pectin (CCP) as a prebiotic, CCP was prepared by the chemical method of sodium hydroxide-chloroacetic acid, and the structure was characterized by infrared spectroscopy.Its anti-digestibility was explored by simulating the human digestive environment in this study, which used CCP as a carbon source to study its prebiotic effects on Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus delbrueckii subsp.bulgaricus, and Streptococcus thermophilus.Results showed that CCP had characteristic absorption peaks at 1 616, 1 419, and 1 313 cm-1, which indicated that it was successfully modified and the degree of substitution was 0.437 1.In the simulated human anti-digestion experiment, the hydrolysis degree of CCP in the simulated saliva, gastric juice, and intestinal juice was lower than 3%, 6%, and 2%, respectively, indicating its resistance to digestion in the human digestive tract.The optimal mass concentration of CCP for promoting the proliferation of Lactobacillus brevis was 15 g/L, and for the other three probiotics was 30 g/L.Under the optimal mass concentration, the probiotics reached the stable period of proliferation in 18-30 h, the bacterial concentration reached the highest value, and the pH value of the medium reached the lowest value.The CCP was superior to citrus pectin in promoting the proliferation of four intestinal probiotics.CCP has the potential to be used as prebiotics and has high development potential in the fields of medicine and health care.
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