Effects of zinc sulfate stress on functional components and antioxidant capacity of germinating barley

  • XU Ningli ,
  • TAO Jin ,
  • ZHANG Lifang ,
  • ZHANG Guoqiang
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  • (College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, China)

Received date: 2023-01-17

  Revised date: 2023-03-21

  Online published: 2024-06-11

Abstract

The study used Zangqing 2000 as the experimental material, the content of functional components and the antioxidant capacity of germinating barley under different concentrations of zinc sulfate stress were determined by enzyme-linked immunosorbent and other approaches, meanwhile, with the germination rate and other indicators.Results showed that zinc sulfate stress inhibited germination rate, moisture content, root length and bud length of barley.Under different concentrations of zinc sulfate stress, the contents of melatonin and γ-aminobutyric acid increased and then decreased.The contents of reducing sugars and soluble sugars increased and then reached a dynamic balance, and the contents of β-glucan, total flavonoids, and total phenols decreased and then increased with the extension of treatment time.In the results, total phenolic content at 7 days of 0.6 mmol/L zinc sulfate stress sprouting was 1.12 times higher than that of the control, melatonin and γ-aminobutyric acid contents at 6 days of 0.8 mmol/L zinc sulfate stress sprouting were 1.35 and 1.40 times higher than those of the control, respectively, and total flavonoid content at 7 days was 1.28 times higher than that of the control.The results of the study on antioxidant capacity showed that the antioxidant capacity of germinating barley was significantly correlated with its total flavonoid and total phenolic content (P<0.01).Taken together, germination can be performed to improve the quality of barley, while zinc sulfate stress at 0.6-0.8 mmol/L can effectively increase the content of functional components and the antioxidant capacity of germinated barley.

Cite this article

XU Ningli , TAO Jin , ZHANG Lifang , ZHANG Guoqiang . Effects of zinc sulfate stress on functional components and antioxidant capacity of germinating barley[J]. Food and Fermentation Industries, 2024 , 50(8) : 114 -120 . DOI: 10.13995/j.cnki.11-1802/ts.034922

References

[1] ZONG Y, TIAN S Q, ZHANG Y, et al. Effects of highland barley powders with different peeling rates on the rheological properties of dough and sensory, volatile flavor evaluation of bread[J]. Journal of Food Processing and Preservation, 2022, 46(11): e16971.
[2] 黄海皎, 李杨, 高小丽, 等. 西藏地区青稞籽粒营养品质分析及评价[J]. 麦类作物学报, 2020, 40(12):1479-1485.
HUANG H J, LI Y, GAO X L, et al. Analysis and evaluation of grain nutritional quality of the highland hulless barley in Tibet[J]. Journal of Triticeae Crops, 2020, 40(12):1479-1485.
[3] 鲁朝凤, 黄佳琦, 黄勇桦, 等. 青稞膳食纤维和多酚对肠道微生物的协同调节作用[J]. 食品与发酵工业, 2021, 47(8):6-13.
LU C F, HUANG J Q, HUANG Y H, et al. Synergetic regulation of gut microbiota by dietary fiber and phenolic compounds in hulless barley[J]. Food and Fermentation Industries, 2021, 47(8):6-13.
[4] DENG N, ZHENG B S, LI T, et al. Assessment of the phenolic profiles, hypoglycemic activity, and molecular mechanism of different highland barley (Hordeum vulgare L.) varieties[J]. International Journal of Molecular Sciences, 2020, 21(4):1175.
[5] 周一鸣, 王宏, 崔琳琳, 等. 萌发苦荞淀粉的理化特性及消化性研究[J]. 中国粮油学报, 2017, 32(3):25-29; 35.
ZHOU Y M, WANG H, CUI L L, et al. Physicochemical properties and digestibility of germination of buckwheat starch[J]. Journal of the Chinese Cereals and Oils Association, 2017, 32(3):25-29; 35.
[6] CHO D H, LIM S T. Germinated brown rice and its bio-functional compounds[J]. Food Chemistry, 2016, 196:259-271.
[7] WEI Y Y, SHOHAG M J I, WANG Y Y, et al. Effect of zinc sulfate fortification in germinated brown rice on seed zinc concentration, bioavailability, and seed germination[J]. Journal of Agricultural and Food Chemistry, 2012, 60(7):1871-1879.
[8] ARNAO M B, HERNÁNDEZ-RUIZ J. Chemical stress by different agents affects the melatonin content of barley roots[J]. Journal of Pineal Research, 2009, 46(3):295-299.
[9] ZHANG Y, FU C X, YAN Y J, et al. Zinc sulfate and sugar alcohol zinc sprays at critical stages to improve apple fruit quality[J]. HortTechnology, 2013, 23(4):490-497.
[10] 朱雪丰. 硫酸锌施用量对桔梗生长和生理特性的影响[D]. 大庆: 黑龙江八一农垦大学, 2022.
ZHU X F. Effect of zinc sulfate application on the growth and physiological characteristics of platycodon grandiflorum[D]. Daqing: Heilongjiang Bayi Agricultural University, 2022.
[11] CAO M L. Effects of spraying zinc fertilizer on the physiological and photosynthetic characteristics of millet plants (Setaria italica L.) at different growth stages[J]. Applied Ecology and Environmental Research, 2019, 17(4): 8121-8138.
[12] 赵爽, 周羽琪, 杨旭妍, 等. 硒硫互作对不结球大白菜芽苗菜硫苷含量及抗氧化性影响[J/OL]. 食品科学, 2022:1-12. https://kns.cnki.net/kcms/detail/11.2206.ts.20220509.1605.004.html.
ZHAO S, ZHOU Y Q, YANG X Y, et al. Effect of selenium and sulfur interaction on the content of glucosinolate and antioxidant activity in sprouts of non-heading Chinese cabbage[J/OL]. Food Science, 2022:1-12. https://kns.cnki.net/kcms/detail/11.2206.ts.20220509.1605.004.html.
[13] 梁雨荷, 党斌, 杨希娟, 等. 萌发青稞营养成分、多酚含量及抗氧化活性研究[J]. 食品科学技术学报, 2019, 37(2):70-81.
LIANG Y H, DANG B, YANG X J, et al. Study on changes of nutrients, polyphenol contents, and antioxidant activities of germinated hulless barley[J]. Journal of Food Science and Technology, 2019, 37(2):70-81.
[14] 曾晴, 谢菲, 尹京苑, 等. 大豆发芽富集γ-氨基丁酸的培养液组分优化及盐胁迫下的富集机理[J]. 食品科学, 2017, 38(12):96-103.
ZENG Q, XIE F, YIN J Y, et al. Optimization of medium composition for γ-aminobutyric acid accumulation in germinated soybean and mechanism of γ-aminobutyric acid accumulation under salt stress[J]. Food Science, 2017, 38(12):96-103.
[15] 朱雪洋, 孟想, 徐宁莉, 等. 光照对黑青稞萌发过程中褪黑素、酚类物质含量和抗氧化活性的影响[J]. 麦类作物学报, 2021, 41(12):1503-1511.
ZHU X Y, MENG X, XU N L, et al. Effect of illumination on melatonin, phenolic content and antioxidant activity during germination of black highland barley[J]. Journal of Triticeae Crops, 2021, 41(12):1503-1511.
[16] 高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006: 145-146.
GAO J F. Experimental Guidance of Plant Physiology[M]. Beijng: Higher Education Press, 2006: 145-146.
[17] WANG J F, BIAN Z X, WANG S M, et al. Effects of ultrasonic waves, microwaves, and thermal stress treatment on the germination of Tartary buckwheat seeds[J]. Journal of Food Process Engineering, 2020, 43(10): e13494.
[18] SHEN Y B, HU C R, ZHANG H, et al. Characteristics of three typical Chinese highland barley varieties: Phenolic compounds and antioxidant activities[J]. Journal of Food Biochemistry, 2018, 42(2): e12488.
[19] 孟想, 朱雪洋, 张莉方, 等. 萌发处理对黑青稞活性成分组成及抗氧化能力的影响[J]. 食品与发酵工业, 2022, 48(4):158-164.
MENG X, ZHU X Y, ZHANG L F, et al. Effect of germination on the composition of active ingredients and antioxidant capacity in black highland barley[J]. Food and Fermentation Industries, 2022, 48(4):158-164.
[20] BACK K, TAN D X, REITER R J. Melatonin biosynthesis in plants: Multiple pathways catalyze tryptophan to melatonin in the cytoplasm or chloroplasts[J]. Journal of Pineal Research, 2016, 61(4):426-437.
[21] ZHANG G Q, YAN Y Y, ZENG X Q, et al. Quantitative proteomics analysis reveals proteins associated with high melatonin content in barley seeds under NaCl-induced salt stress[J]. Journal of Agricultural and Food Chemistry, 2022, 70(27):8492-8510.
[22] 李婷玉, 杜艳, 陈正行, 等. 胁迫萌发对青稞籽粒中β-葡聚糖和γ-氨基丁酸含量的影响[J]. 中国粮油学报, 2021, 36(6):30-35.
LI T Y, DU Y, CHEN Z X, et al. Effects of highland barley seed germination under stress on contents of β-glucan and γ-aminobutyric acid[J]. Journal of the Chinese Cereals and Oils Association, 2021, 36(6):30-35.
[23] GIANINETTI A. A theoretical framework for beta-glucan degradation during barley malting[J]. Theory in Biosciences, 2009, 128(2):97-108.
[24] ELBALOULA M F, HASSAN A B. Effect of different salt concentrations on the gamma-aminobutyric-acid content and glutamate decarboxylase activity in germinated Sorghum (Sorghum bicolor L. Moench) grain[J]. Food Science & Nutrition, 2022, 10(6):2050-2056.
[25] SHELP B J, BOZZO G G, TROBACHER C P, et al. Hypothesis/review: Contribution of putrescine to 4-aminobutyrate (GABA) production in response to abiotic stress[J]. Plant Science, 2012, 193-194:130-135.
[26] 李有媛, 赵愉涵, 陈庆敏, 等. 四种芹菜不同部位营养成分和抗氧化能力的分析比较[J]. 食品与发酵工业, 2021, 47(14):76-81.
LI Y Y, ZHAO Y H, CHEN Q M, et al. Analysis and comparison of nutritional components and antioxidant capacity in different parts of four kinds of celery[J]. Food and Fermentation Industries, 2021, 47(14):76-81.
[27] ACOSTA-MOTOS J, ORTUÑO M, BERNAL-VICENTE A, et al. Plant responses to salt stress: Adaptive mechanisms[J]. Agronomy, 2017, 7(1):18.
[28] 李世玉. 26份甜瓜材料耐盐性评价及外源NO对甜瓜盐胁迫的缓解效应[D]. 杨凌: 西北农林科技大学, 2022.
LI S Y. Evaluation of 26 melon varieties against salt tolerance and study of mitigating effects by exogenous NO application [D]. Yangling: Northwest A & F University, 2022.
[29] BOUDALI G, GHNAYA T, BEN-ABDALLAH S, et al. Zincum metallicum, a homeopathic drug, alleviates Zn-induced toxic effects and promotes plant growth and antioxidant capacity in Lepidium sativum L[J]. Environmental Science and Pollution Research International, 2022, 29(22):33872-33884.
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