Food and Fermentation Industries >

2024 , Vol. 50 >Issue 8: 138 - 145

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036071

Antibacterial mechanism of Lactobacillus plantarum SCB2505 metabolites against Serratia liquefaciens

  • RAO Wei ,
  • CHEN Kuilin ,
  • FANG Ziying ,
  • CHEN Zhaomin ,
  • FANG Xiang
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  • 1(College of Food Science,South China Agricultural University, Guangzhou 510642, China)
    2(Weyran Food Biotechnology (Shenzhen) Co.Ltd., Shenzhen 518040, China)

Received date: 2023-05-16

  Revised date: 2023-06-05

  Online published: 2024-06-11

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Abstract

Serratia liquefaciens is a common spoilage bacteria and food-borne pathogen in animal-based food during low-temperature storage.In this study, the antibacterial activity of Lactobacillus plantarum SCB2505 metabolites against S.liquefaciens SCB2649 was investigated by measuring the minimum inhibitory concentration (MIC) and growth curve. The composition of antibacterial substances was analyzed by high-temperature heat treatment, catalase and protease treatment, pH adjustment treatment, and lactic acid exclusion experiments.The antibacterial mechanism was studied by measuring the biofilm inhibition rate, the integrity of the cell wall and cell membrane, and the changes in cell morphology.Results showed that the MIC of L.plantarum SCB2505 metabolites against S.liquefaciens was 8 mg/mL.The antibacterial substances were found to well tolerate high temperature, hereby the relative antibacterial activity was 89.09% after heat treatment at 121 ℃.Catalase and protease could reduce the antibacterial activity of metabolites, but the relative antibacterial activity was still greater than 90%.pH adjustment had a significant effect on its antibacterial activity, whereby the lower the pH, the higher the antibacterial activity.However, it was verified that hydrogen ion concentration was not the main factor affecting the antibacterial activity, and it was speculated that the antibacterial substance was a complex of lactic acid, bacteriocin and hydrogen peroxide.The L.plantarum SCB2505 metabolites can inhibit the biofilm synthesis of S.liquefaciens, destroy the integrity of the cell wall and cell membrane, leading to shrinking and sagging of the cells, and even lysing and dead of the cells.These results show that the L.plantarum SCB2505 metabolites have a good application prospect as natural preservatives in the field of animal-based food preservation.

Key words： Lactobacillus plantarum; metabolites; Serratia liquefaciens; antibacterial mechanism; antibacterial activity

Cite this article

RAO Wei , CHEN Kuilin , FANG Ziying , CHEN Zhaomin , FANG Xiang . Antibacterial mechanism of Lactobacillus plantarum SCB2505 metabolites against Serratia liquefaciens[J]. Food and Fermentation Industries, 2024 , 50(8) : 138 -145 . DOI: 10.13995/j.cnki.11-1802/ts.036071

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