Obesity is a widespread disease associated with many serious complications.This study aimed to investigate the potential molecular mechanisms of Torreya seed oil on lipid metabolism in obese mice, an obese mice model established with a high-fat diet to evaluate the hypolipidemic, antioxidant, and anti-inflammatory functions.The levels of total cholesterol, triglycerides, and low-density lipoprotein cholesterol were significantly decreased, but the level of high-density lipoprotein cholesterol was significantly increased in the Torreya seed oil high-dose (SCH) group mice compared with the high-fat diet (HFD) group mice (P<0.05).Meanwhile, Torreya seed oil could significantly reduce weight gain and decrease liver and perirenal fat accumulation.Torreya seed oil also reduced the expression levels of tumor necrosis factor-α and interleukin-6, increased the activities of superoxide dismutase and glutathione peroxidase, and improved oxidative stress and inflammatory response.Real-time quantitative PCR (RT-PCR) showed that the expression level of SPEBP-1c in the Torreya seed oil low-dose group was significantly reduced (P<0.05), while the expression levels of adenosine monophosphate (AMP)-activated protein kinase alpha (AMPKα) and fatty acid synthase (FAS) were only slightly reduced.The expression levels of AMPKα, sterol regulatory element-binding protein-1c (SPEBP-1c), and FAS in the SCH group were significantly decreased compared with those of the untreated obese mice (P<0.05).These results suggested that Torreya seed oil improved lipid metabolism disorders by regulating the AMPKα/SREBP-1c signalling pathway expression, increasing antioxidant levels, and reducing inflammatory responses in obese mice.
JIANG Qihong
,
YAO Shiwei
,
LU Hongling
,
JIANG Chenkai
,
HU Wenjun
,
FENG Yongcai
,
CHEN Pingtan
,
SHEN Guoxin
,
XIANG Xingwei
,
CHEN Lin
. Mechanism of Torreya seed oil on disturbance of lipid metabolism in obese mice[J]. Food and Fermentation Industries, 2024
, 50(8)
: 176
-181
.
DOI: 10.13995/j.cnki.11-1802/ts.035084
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