A sports beverage was developed using small molecular weight hydrolysates (less than 5 kDa) of Nereis succinea.The effects of different auxiliary ingredients, different amounts of small molecular weight hydrolysates in Nereis succinea, and different sterilization methods on the DPPH free radical scavenging activity of the drink were investigated, and the optimal formula for Nereis succinea sports drink was determined by sensory evaluation.The anti-fatigue effect of the Nereis succinea sports drink was also explored.Experimental results showed that the optimal formula for the Nereis succinea sports drink was as follows:40% of the Nereis succinea small molecular weight hydrolysates (less than 5 kDa) content, 5% of white sugar, 0.02% of mogroside polysaccharide, 1.2% of lemon juice, 0.05% of potassium dihydrogen phosphate, 0.1% of sodium dihydrogen phosphate, and 0.05% of NaCl.At a concentration of 2.25 mg/mL, the DPPH free radical scavenging rate was 94.1% and the IC50 value was 0.8 mg/mL.Animal experiments showed that high-dose of Nereis succinea sports drink obviously (P<0.01) prolonged the load swimming time of mice, and low-, medium-, and high-dose of Nereis succinea sports drink significantly increased the content of liver glycogen (P<0.01) and significantly reduced the serum urea nitrogen content of mice (P<0.01).Meanwhile, low-dose of Nereis succinea sports drink obviously reduced the content of blood lactic acid in mice (P<0.01) and showed a significant anti-fatigue effect.
ZHU Guoping
,
CHEN Zihao
,
YE Ning
. Research on sports beverage of Nereis succinea and its effects on anti-fatigue[J]. Food and Fermentation Industries, 2024
, 50(8)
: 204
-209
.
DOI: 10.13995/j.cnki.11-1802/ts.035112
[1] MASON S A, MORRISON D, MCCONELL G K, et al.Muscle redox signalling pathways in exercise.Role of antioxidants[J].Free Radical Biology & Medicine, 2016, 98:29-45.
[2] FAN W W, WAIZENEGGER W, LIN C S, et al.PPAR δ promotes running endurance by preserving glucose[J].Cell Metabolism, 2017, 25(5):1186-1193.
[3] CHEN Y J, KUO C Y, KONG Z L, et al.Anti-fatigue effect of a dietary supplement from the fermented by-products of Taiwan Tilapia aquatic waste and Monostroma nitidum oligosaccharide complex[J].Nutrients, 2021, 13(5):1688.
[4] KIM J H, CHO H D, WON Y S, et al.Anti-fatigue effect of Prunus mume vinegar in high-intensity exercised rats[J].Nutrients, 2020, 12(5):1205.
[5] KIM E K, OH H J, KIM Y S, et al.Purification of a novel peptide derived from Mytilus coruscus and in vitro/in vivo evaluation of its bioactive properties[J].Fish & Shellfish Immunology, 2013, 34 (5):1078-1084.
[6] 余毅豪. 海参肽抗疲劳的作用及机制研究[D].无锡:江南大学, 2021.
YU Y H.Research on anti-fatigue effect and mechanism of sea cucumber peptide[D].Wuxi:Jiangnan University, 2021.
[7] QI B, LIU L, ZHANG H, et al.Anti-fatigue effects of proteins isolated from Panax quinquefolium[J].Journal of Ethnopharmacology, 2014, 153(2):430-434.
[8] 王嘉佳, 李晓倩, 高山, 等.核桃多肽复配核桃乳对小鼠抗疲劳及抗氧化作用研究[J].食品与发酵工业, 2020, 46(19):84-91.
WANG J J, LI X Q, GAO S, et al.Anti-fatigue and antioxidant effects of walnut peptide compound walnut milk on mice[J].Food and Fermentation Industries, 2020, 46(19):84-91.
[9] 朱国萍, 陈自豪, 叶宁.响应面优化沙蚕抗氧化物的超高压提取及其抗氧化活性研究[J].水产科技情报, 2018, 45(5):267-274.
ZHU G P, CHEN Z H, YE N.Optimization of ultra-high pressure extraction technology of antioxidant from Perinereis aibuhitensis using response surface methodology and its antioxidant activity[J].Fisheries Science & Technology Information, 2018, 45(5):267-274.
[10] 刘天红, 王颖, 孙元芹, 等.沙蚕不同酶解产物抗氧化效果研究[J].中国农业科技导报, 2020, 22(1):149-161.
LIU T H, WANG Y, SUN Y Q, et al.Research on the antioxidant effect of different enzymatic hydrolysis products of Nereis succinea[J].Journal of Agricultural Science and Technology, 2020, 22(1):149-161.
[11] 尤久勇. 牡蛎蛋白饮料色泽变化及其控制技术研究[D].湛江:广东海洋大学, 2011.
YOU J Y.The study of color change and control technology for oyster protein beverage[D].Zhanjiang:Guangdong Ocean University, 2011.
[12] 赵璐. 乳酸菌发酵荞麦苗汁工艺优化及其清除DPPH自由基作用研究[D].长春:吉林农业大学, 2014.
ZHAO L.Process optimization of lactic acid bacteria fermented buckwheat sprouts juice and study of its effect on the removal of DPPH free radical[D].Changchun:Jilin Agricultural University, 2014.
[13] 常格. 牡蛎蛋白酶解产物抗疲劳作用研究及新产品研发[D].湛江:广东海洋大学, 2016.
CHANG G.The anti-fatigue effect and of protein hydrolysates from oyster a new related product is investigated[D].Zhanjiang:Guangdong Ocean University, 2016.
[14] HUANG W C, CHIU W C, CHUANG H L, et al.Effect of curcumin supplementation on physiological fatigue and physical performance in mice[J].Nutrients, 2015, 7(2):905-921.
[15] LAMOU B, TAIWE G S, HAMADOU A, et al.Antioxidant and antifatigue properties of the aqueous extract of Moringa oleifera in rats subjected to forced swimming endurance test[J].Oxidative Medicine and Cellular Longevity, 2016, 2016:3517824.
[16] 王雪芹. 鲐鱼多肽的抗氧化活性与抗疲劳作用研究[D].青岛:中国科学院研究生院(海洋研究所), 2014.
WANG X Q.Study on antioxidant activity and antifatigue effect of mackerel polypeptide[D].Qingdao:Institute of Oceanology,Chinese Academy of Sciences, 2014.
[17] LIU Y Y, LIU C J.Anti-fatigue and increasing exercise performance of Actinidia arguta crude alkaloids in mice[J].Journal of Food and Drug Analysis, 2016, 24(4):738-745.
[18] 程兆宇, 房磊.黑米糠抗疲劳运动饮料的研究[J].食品研究与开发, 2015, 36(22):165-168.
CHENG Z Y, FANG L.Study on the antifatigue-sport drink of black rice bran[J].Food Research and Development, 2015, 36(22):165-168.
[19] YANG D S, LIAN J H, WANG L Y, et al.The anti-fatigue and anti-anoxia effects of Tremella extract[J].Saudi Journal of Biological Sciences, 2019, 26(8):2052-2056.
