A method was developed for the determination of amitraz (AMZ), chlordimeform, and their metabolites residues in honey by dispersive solid phase extraction purification-ultra high performance liquid chromatography-tandem.2 g honey sample was fully infiltrated with 10 mL aqueous solution and ultrasonic-assisted extraction with 1% ammonium hydroxide-acetonitrile followed by centrifugation, and then cleaned-up by PSA, C18, and NH2, the target compounds were analyzed by ACQUITY UPLC BEH C18 (2.1 mm×50 mm, 1.7 μm) column using a mixture of methanol and 0.1% formic acid aqueous solution as mobile phases with gradient elution, the mass spectrometer was operated under MRM mode in positive mode.The average recoveries of 6 pesticides and metabolites were in the range of 84.1%-113.8%, and the relative standard deviation (RSD) was in the range of 0.9%-6.0%, the limit of detection was between 0.2 μg/kg and 0.8 μg/kg and the limit of quantification was between 0.7 μg/kg and 2.5 μg/kg for target pesticides, respectively.This method was simple, quick, high sensitivity, and could be used for the routine analysis of amitraz, chlordimeform, and their metabolites residues in honey.
WANG Li
,
WEI Maoqiong
,
KANG Hongyu
,
CHEN Xinglian
,
LAN Shanshan
,
ZHANG Xueyan
,
LIU Hongcheng
. Determination of amitraz, chlordimeform, and their metabolites in honey by dispersive solid phase extraction purification-ultra high performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2024
, 50(8)
: 284
-289
.
DOI: 10.13995/j.cnki.11-1802/ts.036706
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