To improve the stability of gliadin (Gli) nanoparticles (GNPs), the emulsification property of Tremella fuciformis polysaccharides (TFPs) was used to explore the influence of the content of TFPs on the stability of GNPs.The composite nanoparticles of TFPs and Gli (G/T NPs) were prepared by the anti-solvent method.The Z-average, ζ-potential, interaction, and microstructure of the composite carrier particles were analyzed by Zetasizer, Fourier transform infrared spectrometer, and thermal field emission scanning electron microscopy.Results showed G/T NPs were formed by the combination of the electrostatic force and the hydrogen bond force, with the inner GNPs and TFPs wrapped in the outer layer.TFPs could effectively improve the stability of the GNPs system and reduce the aggregation of particles during rotary evaporation.The Z-average, polydispersion index (PDI), and ζ-potential of G/T4∶1 NPs were (198.5±2.6) nm, (0.269±0.005), (36.7±0.6) mV, respectively, and showed pH responsiveness.This study laid the foundation for the subsequent construction of the encapsulation and delivery system of hydrophobic nutrients.
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