Inhibitory effect and mechanism of aloe-emodin on α-glucosidase

  • PAN Ruoyao ,
  • REN Guoyan ,
  • MA Fuli ,
  • PENG Qiyue ,
  • DU Liying
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China)
    2(Henan Engineering Research Center of Food Material, Luoyang 471000, China)
    3(National Demonstration Center for Experimental Food Processing and Safety Education, Luoyang 471000, China)

Received date: 2023-06-29

  Revised date: 2023-07-14

  Online published: 2024-06-11

Abstract

Inhibition of α-glucosidase activity is an effective way to control blood glucose balance.The inhibitory effect of aloe emodin (AE) on α-glucosidase and its mechanism were studied by enzyme activity inhibition experiment, ultraviolet spectroscopy, fluorescence spectrometry, Fourier transform infrared spectroscopy, and molecular docking.Results showed that AE had a significant inhibitory effect on α-glucosidase activity [(IC50=(0.031±0.001) mg/mL)], and the inhibitory type was mixed inhibition.AE bound to α-glucosidase with a binding site by static quenching mechanism.The interaction of AE and α-glucosidase changed the conformation of α-glucosidase, resulting in a decrease in the α-helix, β-turn content and an increase in the β-sheet and random coil content.Further analysis by molecular docking showed that AE formed hydrogen bonds with Thr290, Leu297, Glu296, and Asn2592, formed van der Waals forces with Leu297.AE bound to the hydrophobic binding cavity of α-glucosidase by these intermolecular forces, changing the conformation of the enzyme and reducing enzyme activity.

Cite this article

PAN Ruoyao , REN Guoyan , MA Fuli , PENG Qiyue , DU Liying . Inhibitory effect and mechanism of aloe-emodin on α-glucosidase[J]. Food and Fermentation Industries, 2024 , 50(10) : 134 -140 . DOI: 10.13995/j.cnki.11-1802/ts.036611

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