In this study, Luminol was co-blended into β-glucan to prepare an ammonia-responsive β-glucan-Luminol fluorescent colorimetric tablet to monitor the freshness of chilled pork by an infrared compression machine.The structure and fluorescence properties of the material were characterized by Fourier transform infrared spectroscopy, fluorescence emission and fluorescence excitation spectroscopy.Four concentrations of ammonia were used to simulate the ammonia produced during the spoilage of chilled pork.Under 365 nm UV irradiation, the fluorescent colorimetric tablets showed a fluorescence quenching after the dropwise addition of hydrogen peroxide, and the fluorescence gradually decreased to quenching after the dropwise addition of hydrogen peroxide at pH 8-14.When it was used as the indicator of the freshness of chilled pork during storage, the volatile salt nitrogen of chilled pork was 23.6 mg/100 g on the 8th day of storage, which exceeded the national standard.Accordingly, the B value of the colorimetric tablets was significantly lower (P<0.05).Furthermore, the fluorescence changes of the colorimetric tablets established a significant link with a total bacterial colony, volatile saline nitrogen and pH, indicating that fluorescent colorimetric tablets could be used for the monitoring of the freshness of chilled pork.
[1] BAĞDATLI A, KAYAARDI S.Influence of storage period and packaging methods on quality attributes of fresh beef steaks[J].CyTA-Journal of Food, 2015, 13(1):124-133.
[2] BELOTTI M, EL-TAHAWY M M T, DARWISH N, et al.Electrochemically generated luminescence of luminol and luciferin in ionic liquids[J].ChemElectroChem, 2023, 10(1):e202201033.
[3] 杨步高, 赵一, 韦晓菲, 等.一种基于异硫氰酸荧光素的pH纳米传感器制备[J].发光学报, 2020, 41(6):729-733.
YANG B G, ZHAO Y, WEI X F, et al.Preparation of fluorescent pH nanosensor based on flourescein isothiocyanate[J].Chinese Journal of Luminescence, 2020, 41(6):729-733.
[4] 仝坤, 张以河, 谢加才, 等.提高稠油废水可生化性的研究进展[J].化工环保, 2011, 31(5):423-426.
TONG K, ZHANG Y H, XIE J C, et al.Research progresses in biodegradability improvement of viscous oil wastewater[J].Environmental Protection of Chemical Industry, 2011, 31(5):423-426.
[5] 张红莲, 夏立群, 段东霞, 等.含油废水中絮凝剂产生菌DX-3的分离鉴定及特性研究[J].化学与生物工程, 2011, 28(11):22-26.
ZHANG H L, XIA L Q, DUAN D X, et al.Study on isolation, identification and characteristics of a flocculant-producing bacterium DX-3 from oil-containing wastewater[J].Chemistry & Bioengineering, 2011, 28(11):22-26.
[6] NAWAZ H, CHEN S, ZHANG X, et al.Cellulose-based fluorescent material for extreme pH sensing and smart printing applications[J].ACS Nano, 2023, 17(4):3996-4008.
[7] YUAN S J, YU R, TU Y, et al.An enhanced chemiluminescence hybrids of luminol by sulfonated polyaniline decorated copper-based metal organic frame composite applicable to the measurement of hydrogen peroxide in a wide pH range[J].Talanta, 2023, 254:124183.
[8] WANG L, YE F Y, LI S, et al.Effects of oat β-glucan incorporation on the gelatinization, flowability and moisture sorption of wheat flour[J].Powder Technology, 2017, 315:430-437.
[9] CHEN H Z, ZHANG M, BHANDARI B, et al.Novel pH-sensitive films containing curcumin and anthocyanins to monitor fish freshness[J].Food Hydrocolloids, 2020, 100:105438.
[10] XU L F, CAO J G, ZHONG S L, et al.Seeking aggregation-induced emission materials in food:Oat β-glucan and its diverse applications[J].Journal of Agricultural and Food Chemistry, 2021, 69(27):7680-7686.
[11] HALLAJ T, AMJADI M, MIRBIRANG F.S, N-doped carbon quantum dots enhanced Luminol-Mn (Ⅳ) chemiluminescence reaction for detection of uric acid in biological fluids[J].Microchemical Journal, 2020, 156:104841.
[12] 张一平, 邓昆月, 赵博轩, 等.鲁米诺的合成与振荡化学发光[J].大学化学, 2022, 37(5):222-232.
ZHANG Y P, DENG K Y, ZHAO B X, et al.Synthesis of luminol and chemiluminescence oscillation[J].University Chemistry, 2022, 37(5):222-232.
[13] 王杨, 隆海燕, 贾曦然.基于人眼色彩差异化感知的图像质量评价研究[J].计算机工程与科学, 2023, 45(2):295-303.
WANG Y, LONG H Y, JIA X R.Research on image quality evaluation based on color differential perception of human eye[J].Computer Engineering & Science, 2023, 45(2):295-303.
[14] 许利峰. 新型天然化合物的发光性能研究[D].长春:吉林大学, 2022.
XU L F.Exploring of luminescence properties of novel natural compounds[D].Changchun:Jilin University, 2022.
[15] 张永芝, 王倩, 赵子豪, 等.葡聚糖的光致发光性质及机理研究[J].高分子学报, 2022, 53(8):906-912.
ZHANG Y Z, WANG Q, ZHAO Z H, et al.Photoluminescence and luminescent mechanism of dextran[J].Acta Polymerica Sinica, 2022, 53(8):906-912.
[16] GIUSSANI A, FARAHANI P, MARTÍNEZ-MUÑOZ D, et al.Molecular basis of the chemiluminescence mechanism of luminol[J].Chemistry A European Journal, 2019, 25(20):5202-5213.
[17] YUSUFU D, MILLS A.Spectrophotometric and digital colour colourimetric (DCC) analysis of colour-based indicators[J].Sensors and Actuators B:Chemical, 2018, 273:1187-1194.
[18] 谭婉碧, 王琴飞, 余厚美, 等.植物源功能活性多糖的提取及其研究进展[J].热带农业科学, 2022, 42(7):90-98.
TAN W B, WANG Q F, YU H M, et al.Research progress, extraction and functional activity of plant polysaccharides[J].Chinese Journal of Tropical Agriculture, 2022, 42(7):90-98.
[19] CUI H, DONG Y, LU T H, et al.Effect of ethanolic extract from Morus alba L.leaves on the quality and sensory aspects of chilled pork under retail conditions[J].Meat Science, 2021, 172:108368.
[20] NERÍN C, TOVAR L, DJENANE D, et al.Stabilization of beef meat by a new active packaging containing natural antioxidants[J].Journal of Agricultural and Food Chemistry, 2006, 54(20):7840-7846.
[21] 尹爱武, 高鹏飞, 蒋佳洁.刺儿茶多酚对冷却猪肉保鲜效果的研究[J].湖南工程学院学报(自然科学版), 2017, 27(2):47-52.
YIN A W, GAO P F, JIANG J J.Fresh-keeping effect of thorn tea polyphenols on chilled pork[J].Journal of Hunan Institute of Engineering(Natural Science Edition), 2017, 27(2):47-52.
