Food and Fermentation Industries >

2024 , Vol. 50 >Issue 10: 312 - 317

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037481

Determination of five nucleotides in infant formula by HPLC based on pass-through solid phase extraction purification

  • HUANG Kun ,
  • ZHANG Ju ,
  • WANG Huixia
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  • (Hubei Provincial Institute for Food Supervision and Test, Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Wuhan 430075, China)

Received date: 2023-09-25

  Revised date: 2023-10-16

  Online published: 2024-06-11

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Abstract

A method for simultaneous determination of five nucleotides (cytidine monophosphate, uridine monophosphate, inosine monophosphate, guanosine monophosphate, and adenosine monophosphate) in infant formula by hydrophilic high performance liquid chromatography was established.The nucleotides in infant formula samples were extracted with ultrapure water and then purified with Oasis PRiME HLB solid-phase extraction column after being deproteinated, separated on a Waters BEH Amide column with a mobile phase consisting of 0.1% (V/V) phosphoric acid-water and acetonitrile by gradient elution,finally determined using diode array detector and quantitated with external standard method.Five nucleotides showed good linearity in the concentration range of 2-40 mg/L with the correlation coefficients greater than 0.999, the limits of detection (LOD) and limits of quantitation (LOQ) were 0.5-1.0 mg/kg and 1.5-3.0 mg/kg, respectively.The average recoveries of blank samples spiked at 6 mg/kg, 15 mg/kg and 30 mg/kg were 90.17%-101.20%, with relative standard deviation (RSD) values of 1.6%-4.5% (n=6).This method proved to be quick, accurate, less matrix interference, highly sensitive and suitable for the simultaneous determination of five nucleotides in infant formula.

Key words： nucleotides; hydrophilic high performance liquid chromatography; infant formula; pass-through solid phase extraction purification

Cite this article

HUANG Kun , ZHANG Ju , WANG Huixia . Determination of five nucleotides in infant formula by HPLC based on pass-through solid phase extraction purification[J]. Food and Fermentation Industries, 2024 , 50(10) : 312 -317 . DOI: 10.13995/j.cnki.11-1802/ts.037481

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