Food and Fermentation Industries >

2024 , Vol. 50 >Issue 9: 9 - 16

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038193

Effects of a functional milk powder on bone health in growing rats

  • YU Mengqi ,
  • HUANG Shuzhen ,
  • LIU Yufei ,
  • CAO Li ,
  • LIU Yan ,
  • ZHANG Lishi ,
  • FENG Haotian ,
  • CHEN Jinyao
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  • 1(West China School of Public Health/West China Fourth Hospital, Sichuan University, Chengdu 610041, China)
    2(Key Laboratory of Food Safety Monitoring and Risk Assessment, Chengdu 610041, China)
    3(National Center of Technology Innovation for Dairy, Hohhot 010080, China)

Received date: 2023-12-07

  Revised date: 2024-01-30

  Online published: 2024-06-11

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Abstract

To evaluate the effects of a functional milk powder added with Drynariae Rhizoma on bone health in growing rats.50 weaned SPF-grade female SD rats were randomly divided into five groups:low-calcium control group, positive control group, low-dose milk powder group, medium-dose milk powder group, and high-dose milk powder group, gavaged for 90 days.A 3-day calcium-balanced experiment was performed before execution.Bilateral femurs were taken for measuring femur dry weights and lengths, bone mineral density, bone calcium levels, tartrate-resistant acid phosphatase staining, and micro-CT scanning.Besides, the serum levels of C-telopeptide of type Ⅰ collagen (CTX-Ⅰ), PINP, and osteocalcin(BGP) were detected.Results have shown that compared with the low-calcium control group, the femur dry weight and femur length (P<0.05, P<0.01), the bone mineral density and bone calcium content (all P<0.01), and the serum levels of CTX-Ⅰ, procollagen type Ⅰ amino-terminal peptide(PINP), and BGP (all P<0.01) significantly increased in the positive control group and all milk powder dose groups;the number of osteoclasts was significantly reduced in all groups except for the low-dose milk power group, among which the PINP/CTX-I ratio in the high-dose milk power group was significantly decreased (P<0.05);except for the low-dose milk powder group, the number of osteoclasts was significantly decreased (P<0.05, P<0.01);the calcium absorption rate and calcium retention rate in the milk powder low and medium dose groups significantly increased (all P<0.01).Micro-CT showed that the bone microstructure of each milk powder dose group was better than that of the low-calcium control group.Thus, the milk powder could improve the bone health of growing rats by prompting bone formation.

Key words： milk powder; Drynariae Rhizoma; bone mineral density; micro-CT; rats

Cite this article

YU Mengqi , HUANG Shuzhen , LIU Yufei , CAO Li , LIU Yan , ZHANG Lishi , FENG Haotian , CHEN Jinyao . Effects of a functional milk powder on bone health in growing rats[J]. Food and Fermentation Industries, 2024 , 50(9) : 9 -16 . DOI: 10.13995/j.cnki.11-1802/ts.038193

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