Food and Fermentation Industries >

2024 , Vol. 50 >Issue 9: 50 - 55

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.036278

Effect of Fe2+ chelating agent on hydroxyproline fermentation

  • GONG Yu ,
  • LI Changgeng ,
  • LI Xu ,
  • XU Qingyang
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  • 1(College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China)
    2(National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin 300457, China)
    3(Tianjin Engineering Lab of Efficient and Green Amino Acid Manufacture, Tianjin 300457, China)

Received date: 2022-05-30

  Revised date: 2022-06-22

  Online published: 2024-06-11

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Abstract

In order to solve the problems of insufficient supply of Fe2+ and low acid production efficiency in the fermentation process of trans-4-hydroxyproline.In this study, Escherichia coli HYP-08 was used as the test strain.Firstly, the stability of ferrous glycinate, ferrous gluconate, ferrous fumarate and their effects on the production of hydroxyproline fermentation were investigated by single factor experiments.Through 5 L fermentation amplification verification, the yield of hydroxyproline, biomass, and sugar acid conversion rate were used as indicators to further explore their effects in the amplification process.The results showed that ferrous gluconate had the best effect on hydroxyproline fermentation.In the fed-batch fermentation production, the feeding method of continuous feeding from 10 h was finally determined, and the biomass and yield of trans-4-hydroxyproline reached the highest.They were 146.2 and 118.6 g/L, respectively, which were 12.1% and 4.2% higher than those before optimization, and the by-product acetic acid was reduced to 1.05 g/L.The addition of ferrous gluconate effectively enhanced the cell viability, improved the yield of hydroxyproline and the conversion rate of sugar and acid, and provided a basis for the production of hydroxyproline by microbial fermentation.

Key words： Fe2+ chelating agent; hydroxyproline; continuous flow; Escherichia coli; ferrous gluconate

Cite this article

GONG Yu , LI Changgeng , LI Xu , XU Qingyang . Effect of Fe2+ chelating agent on hydroxyproline fermentation[J]. Food and Fermentation Industries, 2024 , 50(9) : 50 -55 . DOI: 10.13995/j.cnki.11-1802/ts.036278

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