To determine the suitable storage conditions for fresh daylilies, the purpose of reducing postharvest loss and extending shelf life was achieved.In this study, polyethylene (PE) film packaging with different thicknesses (30 μm, 40 μm, 50 μm) combined with 1-methylcyclopropene (1-MCP) was used to self-regulate fresh daylily.The changes in quality indexes and microstructure of fresh daylily were analyzed under (0±0.5) ℃.Results showed that the combination of film packaging and 1-MCP treatment was an effective way to prolong the freshness life of fresh daylily.Under the condition of (0±0.5) ℃, 50 μm PE film +1-MCP treatment effectively inhibited the increase of respiration intensity and ethylene release rate of fresh daylily, slowed down the decline of hardness and chlorophyll content, maintained the color of fresh daylily and reduced the weight loss rate and decay rate.After storage for 35 days, the respiratory intensity, ethylene release rate, hardness, and chlorophyll content of 50 μm PE film +1-MCP treatment were 61.25 mg CO2/(kg·h), 55.29 μL/(kg·h), 42.41 N and 0.014 9 mg/g, the weight loss rate and decay rate decreased by 0.66% and 80.33% respectively compared with CK treatment.It also inhibited the increase of tissue relative conductivity and malondialdehyde (MDA) content, effectively improved the activities of peroxidase (POD) and catalase (CAT), and inhibited the increase of cellulase (Cx) and β-glucosidase (β-Glu) activities.After storage for 35 days, compared with CK treatment, the tissue relative conductivity and malondialdehyde (MDA) content of samples treated with 50 μm PE film +1-MCP were decreased by 19.18% and 17.51 μg/g, and the POD and CAT activities were as high as 15.05 U/(min·g) and 85.28 U/(min·g), the activities of Cx and β-Glu were as low as 707.86 μg/(h·g) and 209.41 μg/(h·g).The cell morphology of the fresh daylily was better maintained.Among them, 50 μm PE film +1-MCP treatment was the best preservation method for fresh daylily.
[1] 戢得蓉, 杨芳, 杨雯珺, 等.黄花菜贮藏及深加工技术研究进展[J].食品与发酵科技, 2016, 52(2):48-51.
JI D R, YANG F, YANG W J, et al.Review of the research on the storage and processing of daylily[J].Food and Fermentation Technology, 2016, 52(2):48-51.
[2] OU X B, LIU G, WU L H.The complete chloroplast genome of Hemerocallis citrina (Asphodelaceae), an ornamental and medicinal plant[J].Mitochondrial DNA.Part B, Resources, 2020, 5(1):1109-1110.
[3] CHEN Q, FU M R, QU Q L, et al.Effect of blanching pre-treatment on antioxidant activities and involved compounds in fresh daylily (Hemerocallis fulva L.) flowers[J].Quality Assurance and Safety of Crops & Foods, 2015, 7(3):287-293.
[4] FANG Y J, WAKISAKA M.A review on the modified atmosphere preservation of fruits and vegetables with cutting-edge technologies[J].Agriculture, 2021, 11(10):992.
[5] 圣晓婷, 张引引, 罗运君, 等.1-甲基环丙烯结合不同保鲜袋对叶用甘薯茎尖冷藏保鲜效果的影响[J].食品安全质量检测学报, 2019, 10(9):2521-2526.
SHENG X T, ZHANG Y Y, LUO Y J, et al.Effect of 1-methylcyclopropylene combined with different fresh-keeping bags on the cold preservation effect of the stalk eatable sweet potato tips[J].Journal of Food Safety & Quality, 2019, 10(9):2521-2526.
[6] LI J K, LEI H H, SONG H M, et al.1-methylcyclopropene (1-MCP) suppressed postharvest blue mold of apple fruit by inhibiting the growth of Penicillium expansum[J].Postharvest Biology and Technology, 2017, 125:59-64.
[7] WATKINS C B.The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables[J].Biotechnology Advances, 2006, 24(4):389-409.
[8] SITBON C, PALIYATH G.Pre- and Postharvest Treatments Affecting Nutritional Quality[M]//Comprehensive Biotechnology.Amsterdam:Elsevier, 2011:349-357.
[9] 曹建康, 姜微波, 赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社, 2007.
CAO J K, JIANG W B ,ZHAO Y M. Experimental Guidance of Postharvest Physiological Production of Fruits and Vegetables[M]. Beijing China Light Industry Press, 2007.
[10] FARGAŠOVÁ A.Inhibitive effect of organotin compounds on the chlorophyll content of the green freshwater alga Scenedesmus quadricauda[J].Bulletin of Environmental Contamination and Toxicology, 1996, 57(1):99-106.
[11] 高建晓, 古荣鑫, 胡花丽, 等. 不同薄膜包装对黄花菜贮藏品质的影响[J]. 江苏农业科学, 2015, 43(2):255-259.
GAO J X, GU R X, HU H L, et al. Effects of different film packaging on storage quality of day lily[J]. Jiangsu Agricultural Sciences, 2015, 43(2):255-259.
[12] 李合生. 植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000.
LI H S.Experimental Principles and Techniques of Plant Physiology and Biochemistry[M].Beijing:Higher Education Press, 2000.
[13] WANG Y S, TIAN S P, XU Y.Effects of high oxygen concentration on pro- and anti-oxidant enzymes in peach fruits during postharvest periods[J].Food Chemistry, 2005, 91(1):99-104.
[14] ESCALONA V H, VERLINDEN B E, GEYSEN S, et al.Changes in respiration of fresh-cut butterhead lettuce under controlled atmospheres using low and superatmospheric oxygen conditions with different carbon dioxide levels[J].Postharvest Biology and Technology, 2006, 39(1):48-55.
[15] 程赤云, 张娜, 朱喜成, 等.PBAT/PLA复合膜结合1-MCP处理对金玉兰菜保鲜效果的影响[J].食品研究与开发, 2023, 44(6):21-29.
CHENG C Y, ZHANG N, ZHU X C, et al.Effect of PBAT/PLA film combined with 1-MCP on preservation of chicory[J].Food Research and Development, 2023, 44(6):21-29.
[16] 杨万鹏, 马瑞, 杨永义, 等.NaCl处理对黑果枸杞生长、生理指标的影响[J].分子植物育种, 2019, 17(13):4437-4447.
YANG W P, MA R, YANG Y Y, et al.Effects of NaCl treatment on the growth and physiological indexes of Lycium ruthenicum[J].Molecular Plant Breeding, 2019, 17(13):4437-4447.
[17] 王玲, 戴凡炜, 吴继军, 等.自发气调包装对芥蓝采后叶绿素降解及品质保持的影响[J].食品科学技术学报, 2023, 41(1):163-174.
WANG L, DAI F W, WU J J, et al.Effects of modified atmosphere packaging on chlorophyll degradation and quality maintenance of Chinese kale after harvest[J].Journal of Food Science and Technology, 2023, 41(1):163-174.
[18] 贺艳娥. 1-MCP处理对猕猴桃贮藏品质及软化效应的影响[D].杨凌:西北农林科技大学, 2019.
HE Y E.Effects of 1-MCP treatment on quality and softening of kiwifruit during storage[D].Yangling:Northwest A & F University, 2019.
[19] 张中海. 猕猴桃“果锈” 去除方法及其对耐贮性的影响[D].杨凌:西北农林科技大学, 2006.
ZHANG Z H.Removing fruit rust methodes of kiwifruit and influence on storage trait[D].Yangling:Northwest A & F University, 2006.
[20] 阎香言, 张熠可, 李福瑞, 等.不同质地桃果实软化过程中细胞壁组分变化的差异[J].北方园艺, 2017(20):60-66.
YAN X Y, ZHANG Y K, LI F R, et al.Differences in cell wall composition during softening of peach fruit with different flesh texture[J].Northern Horticulture, 2017(20):60-66.
[21] HOLLAND N, NUNES F L, DE MEDEIROS I U D, et al.High-temperature conditioning induces chilling tolerance in mandarin fruit:A cell wall approach[J].Journal of the Science of Food and Agriculture, 2012, 92(15):3039-3045.
[22] 陈新艳. 1-MCP抑制哈密瓜果实采后软化机理的研究[D].乌鲁木齐:新疆农业大学, 2015.
CHEN X Y.Study on inhibiting softening mechanism of postharvest hami cantaloupe by 1-MCP[D].Urumqi:Xinjiang Agricultural University, 2015.
[23] 陈艺晖, 张华, 林河通, 等.1-甲基环丙烯处理对采后杨桃果实软化和细胞壁代谢的影响[J].热带亚热带植物学报, 2013, 21(6):566-571.
CHEN Y H, ZHANG H, LIN H T, et al.Effect of 1-methylcyclopropene(1-MCP) on softening and cell wall metabolism of harvested Averrhoa carambola fruits[J].Journal of Tropical and Subtropical Botany, 2013, 21(6):566-571.
[24] 李瑶. 1-MCP处理结合聚丙烯保鲜盒包装调控金针菇品质劣变机制研究[D].重庆:西南大学, 2018.
LI Y.Study on Mechanism of quality deterioration of flammulina velutipes regulated by 1-MCP treatment combined with polypropylene crisper[D].Chongqing:Southwest University, 2018.
