Neuroprotective and antioxidant effects of Auxis thazard peptides on HT-22 cells

  • LAI Yumei ,
  • JI Hongwu ,
  • CHEN Ming ,
  • ZHANG Di ,
  • SONG Wenkui ,
  • SU Weiming
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution, Zhanjiang 524088, China)
    2(Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China)

Received date: 2023-05-23

  Revised date: 2023-06-13

  Online published: 2024-07-11

Abstract

This study aimed to evaluate the neuroprotective and antioxidant effects of Auxis thazard peptides (ATP) and to study the neuroprotective effect of ATP in the HT-22 cell injury model induced by corticosterone and glutamic acid.The protective effects of ATP on HT-22 injured cells were investigated based on cell morphology, cell viability, cell function, and biochemical indicators.Results showed that the cell viability of HT-22 cells induced by 0.9 mmol/L corticosterone and 15.0 mmol/L glutamic acid for 24 h was about 50%.Under these conditions, 10 μg/mL, 20 μg/mL, and 40 μg/mL ATP could significantly inhibit the decline in HT-22 cell viability induced by corticosterone and glutamic acid, increase the concentrations of serotonin, acetylcholinesterase and choline, reduce acetylcholine activity, inhibit cell damage induced by corticosterone, and inhibit oxidative stress induced by glutamic acid.The activities of superoxide dismutase, glutathione enzyme, and glutathione peroxidase were increased, and the production of oxidation products malondialdehyde and reactive oxygen species was decreased.

Cite this article

LAI Yumei , JI Hongwu , CHEN Ming , ZHANG Di , SONG Wenkui , SU Weiming . Neuroprotective and antioxidant effects of Auxis thazard peptides on HT-22 cells[J]. Food and Fermentation Industries, 2024 , 50(12) : 53 -62 . DOI: 10.13995/j.cnki.11-1802/ts.036226

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