Food and Fermentation Industries >

2024 , Vol. 50 >Issue 12: 308 - 318

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037008

Determination of amino acids by pre-column derivatization-high performance liquid chromatography and nutritional evaluation of Morindae officinalis Radix

  • SHI Juanlan ,
  • FENG Rong ,
  • GAO Weicheng ,
  • HE Lishan ,
  • WANG Xiaoping
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  • 1(Academic Affairs Office, Zhangzhou Health Vocational College, Zhangzhou 363000, China)
    2(Pharmacy Department, Zhangzhou Health Vocational College, Zhangzhou 363000, China)
    3(Pharmacy Department, Xiamen Medical College, Xiamen 361023, China)

Received date: 2023-08-08

  Revised date: 2023-09-11

  Online published: 2024-07-11

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Abstract

This study aimed to establish a pre-column derivatization-high performance liquid chromatography method for the determination of amino acids in Morindae officinalis Radix and evaluate its nutritional value by amino acid ratio coefficient method.The amino acid test solution of Morindae officinalis Radix was prepared by hydrochloric acid hydrolysis method.Phenyl isothiocyanate and triethylamine were used as pre-column derivatization reagents.The amino acid content was determined by high-performance liquid chromatography with Ultimate Amino Acid chromatographic column (4.6 mm×250 mm, 5 μm), 0.1 mol/L anhydrous sodium acetate-acetonitrile as mobile phase, gradient elution, column temperature of 40 ℃, and detection wavelength of 254 nm.The nutritional value of amino acids was evaluated by amino acid ratio (RAA), amino acid ratio coefficient (RC), and ratio coefficient score (SRC).Systematic cluster analysis and principal component analysis were used in classification analysis.The linear relationship of 15 amino acids was good, and the correlation coefficient r≥0.999 6.The total amino acid contents of 16 batches of Morindae officinalis Radix were between 17.87 mg/g and 102.15 mg/g.Fifteen kinds of amino acids were detected in all 16 samples, including 6 kinds of essential amino acids, accounting for 14.15% to 32.06%, and 8 kinds of medicinal amino acids, accounting for 45.20% to 56.31%.The limiting amino acids were threonine and leucine.The amino acid ratio coefficient was between 33.87 and 61.31.The correlation of amino acid composition of Morindae officinalis Radix was significant.Two principal components were proposed in principal component analysis, and the contribution rate of cumulative variance was 97.085%.16 batches of Morindae officinalis Radix are divided into 3 categories.This method was simple and effective.It could effectively separate 15 kinds of amino acids, and had good precision and repeatability.It has high medicinal value and can provided a reference for quality evaluation and resource development of Morindae officinalis Radix.

Key words： Morindae officinalis Radix; pre-column derivatization-high performance liquid chromatography; amino acid; nutritional evaluation; systematic cluster analysis; principal component analysis

Cite this article

SHI Juanlan , FENG Rong , GAO Weicheng , HE Lishan , WANG Xiaoping . Determination of amino acids by pre-column derivatization-high performance liquid chromatography and nutritional evaluation of Morindae officinalis Radix[J]. Food and Fermentation Industries, 2024 , 50(12) : 308 -318 . DOI: 10.13995/j.cnki.11-1802/ts.037008

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