A method was established to simultaneously determine 2′-fucosyllactose (2′-FL) and lactose-N-neotetraose (LNnT) in infant milk powder by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).The sample was extracted by water, precipitated protein, and purified through a needle-type water system filtration membrane.Then the sample solution was separated using an amide column by gradient elution, within acetonitrile and 10 mmol/L ammonium formate aqueous solution (containing 0.1% formic acid, V/V).The selective reaction monitoring mode was used to measure and quantify by external standards.In addition, it also evaluated the solvent effect and matrix effect by dilution method and extraction addition respectively.Results showed that the addition ion peaks [M+NH+4] and [M+H+] of 2′-FL and LNnT in the positive ion mode were better than others.After the gradient conditions and flow rate optimization, both 2′-FL and LNnT could be eluted within 8 min.However, it was found that 2′-FL and LNnT had serious solvent effects, while, in the elimination test, the effect could be eliminated using 50% acetonitrile ammonium formate aqueous solution as the diluted solvent, within 20 times dilution.The linear ranges of 2′-FL and LNnT were 5.0-150.0 ng/mL and 2.5-75.0 ng/mL, respectively.And there was no matrix effect in either 2′-FL or LNnT.The method verification showed that the precision and accuracy parameters of 2′-FL and LNnT met the requirements of the national standard, and this method was suitable for the simultaneous determination of 2′-FL and LNnT in infant formula.
ZHAN Shengqun
,
GE Cheng
,
YAN Li
,
ZHOU Rongjie
,
DING Yuzhen
,
DUAN Xiaoxiao
. Simultaneous determination of 2′-fucosyllactose and lactose-N-neotetraose in infant formula milk powder by ultra-performance liquid chromatography-tandem mass spectrometry[J]. Food and Fermentation Industries, 2024
, 50(12)
: 327
-335
.
DOI: 10.13995/j.cnki.11-1802/ts.037682
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