Food and Fermentation Industries >

2024 , Vol. 50 >Issue 11: 27 - 37

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035686

Molecular mechanism of anti-tumor activity of polysaccharide from Tricholoma lascivum (Fr.) Gillet on S180 tumor-bearing mice

  • ZHAO Nihui ,
  • JIANG Lin ,
  • ZHANG Ding ,
  • DUAN Jiangbo ,
  • DING Xiang ,
  • HOU Yiling
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  • 1(Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, China West Normal University, Nanchong 637009, China)
    2(College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China)

Received date: 2023-03-31

  Revised date: 2023-05-22

  Online published: 2024-07-12

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Abstract

As one of the main functional components of Tricholoma, polysaccharides have activities such as immune regulation, antioxidant and anti-tumor effects.To clarify the structure and the molecular mechanisms of anti-tumor activity of polysaccharide from Tricholoma lascivum (Fr.) Gillet on S180 tumor-bearing mice, the Tricholoma lascivum (Fr.) Gillet polysaccharide (TLG-1) extracted from the fruiting body of Tricholoma lascivum (Fr.) Gillet by hot water extraction method was used as the experimental material, the structure of TLG-1 was preliminarily analyzed, S180 tumor bearing-mice were used as models to detect anti-tumor activity in vivo, the total RNA of S180 tumor tissue was also sequenced using RNA sequencing.Results showed that TLG-1 had the weight-average molecular weight of 13 442 Da and was composed of xylose, mannose, glucose, and galactose in a ratio of 1.57∶1.45∶2.84∶4.14.TLG-1 could significantly inhibit the growth of S180 tumors in mice (P<0.01), with a tumor inhibition rate of 53.8%.Transcriptome sequencing analysis showed that TLG-1 could significantly up-regulate genes such as mt-Co1, Prlr, Foxa1, and down-regulate genes such as Pitx2, Rplp1, Eef1a1, and Lgals1, affecting the metabolic pathways of S180 tumor cells, regulating the cell cycle, and inhibiting epithelial-mesenchymal transition process.Gene ontology enrichment analysis showed that TLG-1 could activate cytokine-mediated signaling pathways and trigger cellular immunity.Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that genes such as Il21, Il12rb2, Il12b, Il2ra, Il2rb, Il2rg, Il15ra, and Ifng in the Jak-STAT signaling pathway were significantly up-regulated.TLG-1 could enhance the anti-tumor immune response of mice by transforming tumor-associated macrophage phenotype, and secreting interferon-γ.The results could provide a theoretical basis for the development and utilization of TLG-1.

Key words： polysaccharide TLG-1; anti-tumor; S180; RNA sequencing; Jak STAT signaling pathway

Cite this article

ZHAO Nihui , JIANG Lin , ZHANG Ding , DUAN Jiangbo , DING Xiang , HOU Yiling . Molecular mechanism of anti-tumor activity of polysaccharide from Tricholoma lascivum (Fr.) Gillet on S180 tumor-bearing mice[J]. Food and Fermentation Industries, 2024 , 50(11) : 27 -37 . DOI: 10.13995/j.cnki.11-1802/ts.035686

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