Food and Fermentation Industries >

2024 , Vol. 50 >Issue 11: 128 - 136

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.035525

Deep eutectic solvent extraction of Phellinus igniarius flavonoids and its hypoglycemic and bile salts-binding abilities

  • WU Yonghua ,
  • HUANG Lili ,
  • WANG Jieyu ,
  • ZHANG Wenli ,
  • CHEN Anhui ,
  • GUO Hongwei ,
  • ZHANG Jianping ,
  • LIU Enqi
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  • 1(Jiangsu Key Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, Xuzhou 221018, China)
    2(Ningbo Yujun Biotechnology Co.Ltd., Ningbo 315000, China)

Received date: 2023-03-20

  Revised date: 2023-05-23

  Online published: 2024-07-12

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Abstract

Seven kinds of deep eutectic solvents (DESs) were first applied to extract Phellinus igniarius flavonoids (PIFs), the extraction conditions were optimized by response surface methodology, the best enrichment resin for flavonoids was selected, and its hypoglycemic and bind bile salts ability in vitro were analyzed.Results showed that the DES composed of glycol and choline chloride was the most suitable for PIFs extraction.The optimum extraction process was as follows, the water content of DES was 30%, the molar ratio of glycol and choline chloride was 8.21∶1, the ratio (mg∶mL) of material to liquid was 50∶1, at 64 ℃ extracting for 43 min, the yield of PIFs was (8.85±0.34)%, which was 2.02 times higher than that of 60% ethanol extraction.The best enrichment resin for PIFs from DES extracts was AB-8, its recovery rate was 85.25%.After purification, the purity of PIFs increased by 6.12 times.Meanwhile, the IC50 values of α-amylase and α-glucosidase inhibition activity were 1.19 mg/mL and 1.28 mg/mL, and the IC50 values of combining capabilities for sodium taurocholate and sodium glycine cholate were 0.62 mg/mL and 1.19 mg/mL, respectively, indicating that the PIFs had a strong hypoglycemic and bind cholate ability.In conclusion, deep eutectic solvent combined with macroporous adsorption resin enrichment can be an alternative method for green and efficient extraction of PIFs.

Key words： deep eutectic solvent; Phellinus igniarius; flavonoids; macroporous adsorption resin; hypoglycemic; bile salts

Cite this article

WU Yonghua , HUANG Lili , WANG Jieyu , ZHANG Wenli , CHEN Anhui , GUO Hongwei , ZHANG Jianping , LIU Enqi . Deep eutectic solvent extraction of Phellinus igniarius flavonoids and its hypoglycemic and bile salts-binding abilities[J]. Food and Fermentation Industries, 2024 , 50(11) : 128 -136 . DOI: 10.13995/j.cnki.11-1802/ts.035525

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