The primary factor limiting athletic performance for athletes and fitness enthusiasts was inadequate exercise endurance.Enhancing exercise endurance not only helps athletes perform better on the field but also helps the general public become more physically healthy.But yet endurance sports functional foods that are safe and efficient for daily consumption are currently unavailable.Yuandongli (YDL), a medicinal and edible compound, has a significant effect on improving exercise endurance, but its mechanism has yet to be further studied systematically.The composition used in this study composed of 28% of allulose, 12% of sucrose, 8% of glucose, 12% of 5-hydroxymethylfurfural, 18% of palmitic acid, 18% of linoleic acid, 3% of ginsenoside, and 1% of gingerol.In this study, mice were administered YDL by gavage.Exercise endurance was determined by a weight-exhaustion swimming test.Blood urea nitrogen (BUN), blood lactate (BLA), liver glycogen (LG), and muscle glycogen (MG) were determined by biochemical methods.Short-chain fatty acids (SCFAs) were determined by GC-MS.The metabolism of mice was monitored by infrared thermography.Morphology of muscle tissue was observed by HE staining.The changes in physiological and biochemical indexes in each group were compared, and the results were that after low and high doses of YDL intervention, the weight-exhaustion swimming time of mice was 119.8% and 257.4% (P<0.05) longer than the blank group, respectively. After low and high-dose YDL intervention, the energy metabolism significantly improved, muscle tissue deformation and rupture reduced, MG content increased, BUN and BLA content decreased, and fecal butyric acid content increased.In summary, YDL can significantly improve energy metabolism, increase butyric acid production, reduce harmful metabolites, increase MG reserves, and improve exercise endurance.
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