The purpose of this paper was to develop a method for the high-throughput determination of furazolidone metabolites, nitrofural metabolites and chloramphenicol drug residues in pork and chicken tissues by colloidal gold immunochromatographic assay (GICA).Furazolidone metabolites, nitrofural metabolites and chloramphenicol drug residues in animal muscle tissues were acidified, derivatized and extracted by ethyl acetate solution and diluted by Tris-HCl buffer with Tween-20, binding to colloidal gold-labeled monoclonal antibodies and inhibiting the binding of antibodies to the antigen on nitrocellulose membrane test line (T-line), which results in a change of the color of T-line.The qualitative determination of poly-veterinary drug residues in the samples was made by comparing the color shades of the T-line and control line (C-line).This test optimized the key factors such as pH value, antibody addition, antigen encapsulation, Tween-20 addition, density of derivatization reagent and type of lyophilized protectant.The results showed that the established immunochromatographic method could detect furazolidone metabolites, nitrofural metabolites, and chloramphenicol in pork and chicken in 35 minutes, and the limit of detection were 0.5, 0.5, 0.1 μg/kg, respectively. The sensitivity ≥98%, the false positive rate ≤2%, the false negative rate ≤2%, the stability can reach 1 year and the method is consistent with the results detected by the existing standard method.The method was simple with strong specificity, high sensitivity, high precision, short detection time and good stability.It could be used for high-throughput rapid detection of multi-veterinary drug residues in animal muscle tissue.
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