β-ionone is a monoterpene compound found in plants that exhibits various biological activities, including deworming, antibacterial, anticancer, and antioxidant properties.It is commonly utilized in agriculture, food, medicine, and flavor industries.Candida tropicalis is a synthetic chassis cell with the potential for efficient terpenoid production.To enhance the yield of β-ionone, based on the β-ionone engineered strain previously constructed by our research group, this study co-expressed PhCCD1, the key enzyme of β-ionone synthesis, in the cytoplasm, peroxisomes, and lipid droplets through subcellular compartment combination of enzymes.This resulted in the generation of β-ionone engineered strain PCL-01.Subsequently, this study optimized the conditions for shaking flask fermentation, including the liquid amount in the medium, the carbon-to-nitrogen ratio, the fermentation temperature, and the concentration of cofactor Fe2+.Finally, this study conducted a small-scale test in the 5 L bioreactor.The shake flask yield of β-ionone for the engineered strain PCL-01 was 152.4 mg/L, representing a 50% increase compared to the starting strain.After optimizing shake flask fermentation, the yield increased by 22% to 185.8 mg/L.Ultimately, in a 5 L bioreactor, the yield reached 403.9 mg/L.This study is significant for the metabolic transformation of other strains in terpenoid synthesis.It also provides valuable references for researching the fermentation performance of other bacterial species.
XU Jie
,
XIA Yuanyuan
,
SHEN Wei
,
YANG Haiquan
,
CHEN Xianzhong
. Construction and fermentation optimization of β-ionone engineered strain in Candida tropicalis[J]. Food and Fermentation Industries, 2024
, 50(13)
: 9
-15
.
DOI: 10.13995/j.cnki.11-1802/ts.039058
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